Search

  • Author merit
Hypo test protocol11
Cell-Intrinsic Barriers of T Cell-Based Immunotherapy.

Author:  hypo 单海波, date: 08/30/2017, view: 574, Q&A: 31
...
More >

这是一条反馈
[Summary 1] 反馈摘要
反馈标题
创建反馈
分享反馈的标题
反馈标题
提交反馈
反馈标题
分享反馈2018-01-18
摘要2
摘要2
反馈
反馈
反馈
反馈一下
反馈意见
提交一条反馈信息
反馈标题q17
feedback
反馈title
提交反馈
反馈
feedback
反馈
反馈2017-12-28 15:04:41
feedback
分享反馈
feedback
测试feedback
测试feedback
share feedback

γ-Secretase Epsilon-cleavage Assay
γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang et al., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are HEK293 cells with a stably integrated luciferase reporter under the control of the bacterial tetO ...
More >


Simple Digital Photography for Assessing Biomass and Leaf Area Index in Cereals
Authors:  Jaume Casadesús and Dolors Villegas, date: 06/05/2015, view: 3998, Q&A: 0
These instructions refer to obtaining fast and low-labour estimates of ground cover, leaf area index and green biomass for a large number of plots, as those encountered in cereal breeding programs. The procedure includes obtaining the pictures in the field and processing them once downloaded to a computer.
More >


Zymogram Assay for the Detection of Peptidoglycan Hydrolases in Streptococcus mutans
Authors:  Delphine Dufour and Céline M. Lévesque, date: 08/20/2013, view: 6039, Q&A: 1
Peptidoglycan hydrolases or autolysins are enzymes capable of cleaving covalent bonds in bacterial peptidoglycan cell wall layer. They can participate in the cell division process, in the release of turnover products from peptidoglycan during cell growth, and in cell autolysis induced under particular conditions. The protocol for zymogram ...
More >

Zymogram tweaking

Chemiluminescence Detection of the Oxidative Burst in Plant Leaf Pieces
Authors:  Markus Albert, Melinka A. Butenko, Reidunn B. Aalen, Georg Felix and Mari Wildhagen, date: 03/20/2015, view: 6104, Q&A: 1
The production of 'reactive oxygen species' (ROS), also termed oxidative burst, is a typical cellular response of animals and plants to diverse biotic and abiotic stresses. Here, we outline the detection of the ROS-burst in plant leaf pieces using a luminol-based bioassay which allows for the detection of chemiluminescence. The assay was ...
More >


Gene Networks Based on the Graphical Gaussian Model
Author:  Shisong Ma, date: 02/20/2012, view: 7684, Q&A: 1
This protocol describes how to build a gene network based on the graphical Gaussian model (GGM) from large scale microarray data. GGM uses partial correlation coefficient (pcor) to infer co-expression relationship between genes. Compared to the traditional Pearson’ correlation coefficient, partial correlation is a better measurement of direct ...
More >

Very good one!

Optical Clearing Using SeeDB
Authors:  Meng-Tsen Ke, Satoshi Fujimoto and Takeshi Imai, date: 02/05/2014, view: 12637, Q&A: 1
We describe a water-based optical clearing agent, SeeDB (See Deep Brain), which clears fixed brain samples in a few days without quenching many types of fluorescent dyes, including fluorescent proteins and lipophilic neuronal tracers. SeeDB is a saturated solution of fructose (80.2% w/w) in water with 0.5% α-thioglycerol. In standard SeeDB optical ...
More >

Testing protocol on random adult mouse brain tissue section

Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
Authors:  Arsalan Daudi and Jose A. O’Brien, date: 09/20/2012, view: 39326, Q&A: 17
In this protocol, the in situ detection of hydrogen peroxide (one of several reactive oxygen species) is described in mature Arabidopsis rosette leaves by staining with 3,3'-diaminobenzidine (DAB) using an adaptation of previous methods (Thordal-Christensen et al., 1997; Bindschedler et al., 2006; Daudi et al. ...
More >

Detection of ROS by DAB and NBT Staining in apples Leaves