Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae
Authors: Zhiqiang Zeng,
Juan C Lopez-Baez,
Laura Lleras-Forero,
Hannah Brunsdon,
Cameron Wyatt,
Witold Rybski,
Nicholas D Hastie,
Stefan Schulte-Merker and
E Elizabeth Patton,
date: 12/05/2018,
view: 86,
Q&A: 0
[Abstract] Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific ...
Zebrafish Embryo Xenograft and Metastasis Assay
[Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...
Medaka-microinjection with an Upright Microscope
Authors: Yu Murakami and
Masato Kinoshita,
date: 02/05/2018,
view: 1501,
Q&A: 0
[Abstract] We described a simple method for microinjecting DNA/RNA/Protein solutions into medaka eggs under an upright microscope. Medaka is an excellent vertebrate model for reverse genetics, because of its daily spawning, short generation time, and egg transparency. These features enable us to efficiently perform functional genomic analyses of transgenic ...
zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
[Abstract] In studies of brain function, it is essential to understand the underlying neuro-architecture. Very young zebrafish larvae are widely used for neuroarchitecture studies, due to their size and natural transparency. However, this model system has several limitations, due to the immaturity, high rates of development and limited behavioral repertoire ...
Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
[Abstract] The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, ...