mRNA Extraction from Gill Tissue for RNA-sequencing
Authors: Jukka-Pekka Verta and
Felicity Jones,
date: 03/05/2020,
view: 286,
Q&A: 0
[Abstract] Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression since divergent adaptation to different environments is common, they are abundant and easy to study ...
Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions
[Abstract] The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host defense against any infection. The zebrafish is an ideal model to study the response of these cells ...
Quantification of Cutaneous Ionocytes in Small Aquatic Organisms
[Abstract] Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can change to help cope with various metabolic and ...
Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae
Authors: Zhiqiang Zeng,
Juan C Lopez-Baez,
Laura Lleras-Forero,
Hannah Brunsdon,
Cameron Wyatt,
Witold Rybski,
Nicholas D Hastie,
Stefan Schulte-Merker and
E Elizabeth Patton,
date: 12/05/2018,
view: 1901,
Q&A: 0
[Abstract] Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific ...