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Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

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Whole-mount Immunohistochemistry of Adult Zebrafish Retina for Advanced Imaging

Authors: Mikiko Nagashima and Peter F. Hitchcock, date: 12/20/2020, view: 764, Q&A: 0

[Abstract]

Immunohistochemistry is a widely used technique to examine the expression and subcellular localization of proteins. This technique relies on the specificity of antibodies and requires adequate penetration of antibodies into tissues. The latter is especially challenging for thick specimens, such as embryos and other whole-mount preparations. Here

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Use of Optogenetic Amyloid-β to Monitor Protein Aggregation in Drosophila melanogaster, Danio rerio and Caenorhabditis elegans

Authors: Prameet Kaur, Caroline Kibat, Emelyne Teo, Jan Gruber, Ajay Mathuru and Nicholas S. Tolwinski, date: 12/05/2020, view: 729, Q&A: 0

[Abstract]

Alzheimer’s Disease (AD) has long been associated with accumulation of extracellular amyloid plaques (Aβ) originating from the Amyloid Precursor Protein. Plaques have, however, been discovered in healthy individuals and not all AD brains show plaques, suggesting that extracellular Aβ aggregates may play a smaller role than

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Advances in Proximity Ligation in situ Hybridization (PLISH)

Authors: Monica Nagendran, Adam M Andruska, Pehr B Harbury and Tushar J Desai, date: 11/05/2020, view: 1209, Q&A: 0

[Abstract] Understanding tissues in the context of development, maintenance and disease requires determining the molecular profiles of individual cells within their native in vivo spatial context. We developed a Proximity Ligation in situ Hybridization technology (PLISH) that enables quantitative measurement of single cell gene expression ...

A Reproducible Protocol to Measure the Critical Swimming Speed of Adult Zebrafish

Authors: Yuma Wakamatsu, Makoto Kashima and Hiromi Hirata, date: 08/20/2020, view: 1265, Q&A: 1

[Abstract] The quantitative measurement of water flow-induced swimming of fish species using a swimmill is a powerful method to evaluate motor ability of individual fish. Zebrafish is a commonly used vertebrate that enables the study of morphological, physiological and behavioral characteristics associated with genes. We here established a reproducible ...

Paper Lateral Flow Biosensor for Nodavirus Reverse Transcribed RNA Detection

Authors: Dimitra K. Toubanaki and Evdokia Karagouni, date: 08/05/2020, view: 897, Q&A: 0

[Abstract] Paper nanobiosensors have been established as an excellent platform for analysis of veterinary and human pathogens causing various diseases. Especially, lateral flow assays or biosensors ideal for sensitive, rapid, robust and accurate analysis in laboratory setups and on-site analysis. Viral RNA detection is of great importance for public health ...

Low-cost and Multiplexable Whole mRNA-Seq Library Preparation Method with Oligo-dT Magnetic Beads for Illumina Sequencing Platforms

Authors: Makoto Kashima, Ayumi Deguchi, Ayumi Tezuka and Atsushi J. Nagano, date: 06/20/2020, view: 2294, Q&A: 0

[Abstract] RNA-Seq is a powerful method for transcriptome analysis used in varied field of biology. Although several commercial products and hand-made protocols enable us to prepare RNA-Seq library from total RNA, their cost are still expensive. Here, we established a low-cost and multiplexable whole mRNA-Seq library preparation method for illumine ...

Quantitative Determination of Ca²⁺-binding to Ca²⁺-sensor Proteins by Isothermal Titration Calorimetry

Authors: Seher Abbas and Karl-Wilhelm Koch, date: 04/05/2020, view: 1714, Q&A: 0

[Abstract] Diverse and complex molecular recognitions are central elements of signal transduction cascades. The strength and nature of these interaction modes can be determined by different experimental approaches. Among those, Isothermal titration calorimetry (ITC) offers certain advantages by providing binding constants and thermodynamic parameters from ...

mRNA Extraction from Gill Tissue for RNA-sequencing

Authors: Jukka-Pekka Verta and Felicity Jones, date: 03/05/2020, view: 1790, Q&A: 0

[Abstract] Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression since divergent adaptation to different environments is common, they are abundant and easy to study ...

Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions

Authors: Faiza Basheer, Clifford Liongue and Alister C Ward, date: 03/05/2020, view: 1671, Q&A: 0

[Abstract] The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host defense against any infection. The zebrafish is an ideal model to study the response of these cells ...

Retina Injury and Retina Tissue Preparation to Study Regeneration in Zebrafish

Authors: Poonam Sharma and Rajesh Ramachandran, date: 12/20/2019, view: 2054, Q&A: 0

[Abstract] Unlike mammals, primitive vertebrates have immense capability to regenerate almost all of their organs including the central nervous system. Among primitive organisms, zebrafish have been extensively used as a model system for regeneration studies. The retina is a part of the central nervous system and mammals lack the potential to repair any ...

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