Neuroscience

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    Protocols in Current Issue
    Activity-based Anorexia for Modeling Vulnerability and Resilience in Mice
    Authors:  Jeff A. Beeler and Nesha S. Burghardt, date: 05/05/2021, view: 203, Q&A: 0

    Activity-based anorexia (ABA) is a widely used rodent model of anorexia nervosa. It involves combining limited access to food with unlimited access to a running wheel, leading to a paradoxical decrease in food intake, hyperactivity, and

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    Activity-based Anorexia for Modeling Vulnerability and Resilience in Mice
    Authors:  Jeff A. Beeler and Nesha S. Burghardt, date: 05/05/2021, view: 203, Q&A: 0
    [Abstract]

    Activity-based anorexia (ABA) is a widely used rodent model of anorexia nervosa. It involves combining limited access to food with unlimited access to a running wheel, leading to a paradoxical decrease in food intake, hyperactivity, and life-threatening weight loss. Although initially characterized in rats, ABA has been tested in mice with results

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    A Method to Quantify Drosophila Behavioral Activities Induced by GABAA Agonist Muscimol
    Author:  M. Rezaul Karim, date: 04/20/2021, view: 421, Q&A: 0
    [Abstract]

    Muscimol is a psychoactive isoxazole derived from the mushroom Amanita muscaria. As a potent GABAA receptor agonist, muscimol suppresses the activity of the central nervous system, reduces anxiety and induces sleep. We investigated the effects of muscimol on Drosophila behavior. Drosophila behavioral assays are powerful tools that are used to

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    Single or Repeated Ablation of Mouse Olfactory Epithelium by Methimazole
    Authors:  Sofia Håglin, Staffan Bohm and Anna Berghard, date: 04/20/2021, view: 495, Q&A: 0
    [Abstract]

    Odor-detecting olfactory sensory neurons residing in the nasal olfactory epithelium (OE) are the only neurons in direct contact with the external environment. As a result, these neurons are subjected to chemical, physical, and infectious insults, which may be the underlying reason why neurogenesis occurs in the OE of adult mammals. This feature

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    Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells
    Authors:  Adam Sivitilli, Parisa Ghiasi and Liliana Attisano, date: 04/20/2021, view: 701, Q&A: 0
    [Abstract]

    Recent advances in stem cell technology have allowed researchers to generate 3D cerebral organoids (COs) from human pluripotent stem cells (hPSCs). Indeed, COs have provided an unprecedented opportunity to model the developing human brain in a 3D context, and in turn, are suitable for addressing complex neurological questions by leveraging

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    Single-unit Recording in Awake Behaving Non-human Primates
    Authors:  Mengxi Yun, Masafumi Nejime and Masayuki Matsumoto, date: 04/20/2021, view: 467, Q&A: 0
    [Abstract]

    Non-human primates (NHPs) have been widely used as a species model in studies to understand higher brain functions in health and disease. These studies employ specifically designed behavioral tasks in which animal behavior is well-controlled, and record neuronal activity at high spatial and temporal resolutions while animals are performing the

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    Transfection and Activation of CofActor, a Light and Stress Gated Optogenetic Tool, in Primary Hippocampal Neuron Cultures
    Authors:  Wyatt P. Bunner, Rachel Dodson, Robert M. Hughes and Erzsebet M. Szatmari, date: 04/20/2021, view: 505, Q&A: 0
    [Abstract]

    Proteins involved in neurodegeneration can be coupled with optogenetic reagents to create rapid and sensitive reporters to provide insight into the biochemical processes that mediate the progression of neurodegenerative disorders, including Alzheimer’s Disease (AD). We have recently developed a novel optically-responsive tool (the

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    Development of a Chemical Reproductive Aging Model in Female Rats
    [Abstract]

    Women are born with an abundant but finite pool of ovarian follicles, which naturally and progressively decreased during their reproductive years until menstrual periods stop permanently (menopause). Perimenopause represents the transition from reproductive to non-reproductive life. It is usually characterized by neuroendocrine, metabolic and

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    Imaging Microtubules in vitro at High Resolution while Preserving their Structure
    Authors:  Camille Cuveillier, Yasmina Saoudi, Isabelle Arnal and Christian Delphin, date: 04/05/2021, view: 336, Q&A: 0
    [Abstract]

    Microtubules (MT) are the most rigid component of the cytoskeleton. Nevertheless, they often appear highly curved in the cellular context and the mechanisms governing their overall shape are poorly understood. Currently, in vitro microtubule analysis relies primarily on electron microscopy for its high resolution and Total Internal Reflection

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    Cranioplastic Surgery and Acclimation Training for Awake Mouse fMRI
    Authors:  Tomokazu Tsurugizawa, Kota Tamada, Clement Debacker, Andrew Zalesky and Toru Takumi, date: 04/05/2021, view: 324, Q&A: 0
    [Abstract]

    MRI is a promising tool for translational research to link brain function and structure in animal models of disease to patients with neuropsychiatric disorders. However, given that mouse functional MRI (fMRI) typically relies on anesthetics to suppress head motion and physiological noise, it has been difficult to directly compare brain fMRI in

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    Ligand and Carbohydrate Engagement (LACE) Assay and Fluorescence Quantification on Murine Neural Tissue
    Authors:  James M. Clegg and Thomas Pratt, date: 03/20/2021, view: 503, Q&A: 0
    [Abstract]

    The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and carbohydrate engagement (LACE) assay is a powerful tool which can be used to probe the molecular interaction

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