Neuroscience

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    Protocols in Current Issue
    An Alternative Maze to Assess Novel Object Recognition in Mice
    The novel object recognition (NOR) task is a behavioral test commonly used to evaluate episodic-like declarative memory and it relies on the innate tendency of rodents to explore novelty. Here we present a maze used to evaluate NOR memory in mice ...
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and ...
    An Alternative Maze to Assess Novel Object Recognition in Mice
    [Abstract] The novel object recognition (NOR) task is a behavioral test commonly used to evaluate episodic-like declarative memory and it relies on the innate tendency of rodents to explore novelty. Here we present a maze used to evaluate NOR memory in mice that reduces the time of the assay while improving reliability of the measurements by increasing the ...
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    [Abstract] Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to ...
    Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers
    Authors:  Xue Mei Song, Ming Li, Tao Xu, Dewen Hu and Anna Wang Roe, date: 06/05/2020, view: 330, Q&A: 0
    [Abstract] In the mammalian visual system, early stages of visual form perception begin with orientation selective neurons in primary visual cortex (V1). In many species (including humans, monkeys, tree shrews, cats, and ferrets), these neurons are organized in pinwheel-like orientation columns. To study the functional organization within orientation ...
    Enriched Environment Procedures for Rodents: Creating a Standardized Protocol for Diverse Enrichment to Improve Consistency across Research Studies
    Authors:  Bruce C. Harland and John C. Dalrymple-Alford, date: 06/05/2020, view: 360, Q&A: 0
    [Abstract] Exposure to environmental enrichment has beneficial effects on learning and memory, diverse neurobiological effects, and promotes recovery of function after brain injury. The effect of enrichment is produced by a combination of increased social interaction, physical activity, spatial complexity, and novelty. Procedures in the literature have, ...
    Generation, Analyzing and in-vivo Drug Treatment of Drosophila Models with IBMPFD
    Authors:  Ting Zhang, Bruce A. Hay and Ming Guo, date: 05/20/2020, view: 480, Q&A: 0
    [Abstract] Missense mutations of p97/cdc48/Valosin-containing protein (VCP) cause inclusion body myopathy, Paget disease with frontotemporal dementia (IBMPFD) and other neurodegenerative diseases. The pathological mechanism of IBMPFD is not clear and there is no treatment. We generated Drosophila models of IBMPFD in adult flight muscle in ...
    Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies
    [Abstract] CRISPR-Cas9 technology has transformed the ability to edit genomic sequences and control gene expression with unprecedented ease and scale. However, precise genomic insertions of coding sequences using this technology remain time-consuming and inefficient because they require introducing adjacent single-strand cuts through Cas9 nickase action and ...
    Examining Cocaine Conditioning Place Preference in Mice
    Authors:  MaryElizabeth J. Simkevich, Rianne R. Campbell and André O. White, date: 04/20/2020, view: 546, Q&A: 0
    [Abstract] A key component of combating substance use disorders is understanding the neural mechanisms that support drug reward. Tasks such as self-administration assess the reinforcing properties of a drug using a learned behavior but require numerous training sessions and surgery. In comparison, the conditioned place preference (CPP) task assesses reward ...
    High-throughput Flow Cytometry Assay to Investigate TDP43 Splicing Function
    Authors:  H. Broder Schmidt and Rajat Rohatgi, date: 04/20/2020, view: 876, Q&A: 0
    [Abstract] Mutations in RNA-binding proteins (RBPs) such as TDP43 are associated with transcriptome-wide splicing defects and cause severe neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The impact of RBP mutations on splicing function is routinely studied using PCR-based bulk measurements. ...
    Minimally Invasive Oral Surgery Induction of the FRICT-ION Chronic Neuropathic Pain Model
    Authors:  Marena A. Montera and Karin N. Westlund, date: 04/20/2020, view: 518, Q&A: 0
    [Abstract] An easily induced preclinical trigeminal neuropathic nerve injury model is described here for the study of chronic pain, the model acronym FRICT-ION (Foramen Rotundum Inflammatory Constriction Trigeminal InfraOrbital Nerve). In patients, neuropathic pain is thought to be related to ...
    A Simple and Fast Battery Test for Phenotypic Characterization of Mice
    [Abstract] Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or ...



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