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    Protocols in Current Issue
    Assaying Thermo-nociceptive Behavior in Drosophila Larvae
    Authors:  Meike Petersen, Federico Tenedini, Nina Hoyer, Fritz Kutschera and Peter Soba, date: 02/20/2018, view: 24, Q&A: 0
    Thermo-nociception, the detection and behavioral response to noxious temperatures, is a highly conserved action to avoid injury and ensure survival. Basic molecular mechanisms of thermal responses have been elucidated in several model organisms and ...
    Assaying Mechanonociceptive Behavior in Drosophila Larvae
    Authors:  Nina Hoyer, Meike Petersen, Federico Tenedini and Peter Soba, date: 02/20/2018, view: 18, Q&A: 0
    Drosophila melanogaster larvae have been extensively used as a model to study the molecular and cellular basis of nociception. The larval nociceptors, class IV dendritic arborization (C4da) neurons, line the body wall of the animal and ...
    Registration and Alignment Between in vivo Functional and Cytoarchitectonic Maps of Mouse Visual Cortex
    Authors:  Jun Zhuang, Quanxin Wang, Marc Takeno and Jack Waters, date: 02/20/2018, view: 21, Q&A: 0
    This protocol describes a method for registration of in vivo cortical retinotopic map with cytochrome c oxidase (CO) labeled architectonic maps of the same mouse brain through the alignment of vascular fiducials. By recording surface blood ...
    Plate Assay to Determine Caenorhabditis elegans Response to Water Soluble and Volatile Chemicals
    Authors:  Takashi Murayama and Ichiro N. Maruyama, date: 02/20/2018, view: 21, Q&A: 0
    The nematode Caenorhabditis elegans is widely used for behavioral studies ranging from simple chemosensation to associative learning and memory. It is vital for such studies to determine optimal concentrations of attractive and aversive ...
    Assaying Thermo-nociceptive Behavior in Drosophila Larvae
    Authors:  Meike Petersen, Federico Tenedini, Nina Hoyer, Fritz Kutschera and Peter Soba, date: 02/20/2018, view: 24, Q&A: 0
    [Abstract] Thermo-nociception, the detection and behavioral response to noxious temperatures, is a highly conserved action to avoid injury and ensure survival. Basic molecular mechanisms of thermal responses have been elucidated in several model organisms and are of clinical relevance as thermal hypersensitivity (thermos-allodynia) is common in neuropathic ...
    Assaying Mechanonociceptive Behavior in Drosophila Larvae
    Authors:  Nina Hoyer, Meike Petersen, Federico Tenedini and Peter Soba, date: 02/20/2018, view: 18, Q&A: 0
    [Abstract] Drosophila melanogaster larvae have been extensively used as a model to study the molecular and cellular basis of nociception. The larval nociceptors, class IV dendritic arborization (C4da) neurons, line the body wall of the animal and respond to various stimuli including noxious heat and touch. Activation of C4da neurons results in a ...
    Registration and Alignment Between in vivo Functional and Cytoarchitectonic Maps of Mouse Visual Cortex
    Authors:  Jun Zhuang, Quanxin Wang, Marc Takeno and Jack Waters, date: 02/20/2018, view: 21, Q&A: 0
    [Abstract] This protocol describes a method for registration of in vivo cortical retinotopic map with cytochrome c oxidase (CO) labeled architectonic maps of the same mouse brain through the alignment of vascular fiducials. By recording surface blood vessel pattern and sequential alignment at each step, this method overcomes the challenge imposed by ...
    Plate Assay to Determine Caenorhabditis elegans Response to Water Soluble and Volatile Chemicals
    Authors:  Takashi Murayama and Ichiro N. Maruyama, date: 02/20/2018, view: 21, Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is widely used for behavioral studies ranging from simple chemosensation to associative learning and memory. It is vital for such studies to determine optimal concentrations of attractive and aversive chemicals that C. elegans can sense. Here we describe a resource localization assay in which a ...
    Culture and Nucleofection of Postnatal Day 7 Cortical and Cerebellar Mouse Astroglial Cells
    Authors:  Malek Chouchane and Marcos Romualdo Costa, date: 02/05/2018, view: 180, Q&A: 0
    [Abstract] Lineage reprogramming of astroglial cells isolated from different brain regions leads to the generation of different neuronal subtypes. This protocol describes the isolation and culture of neocortical and cerebellar astrocytes from postnatal mice. We also present a comprehensive description of the main steps towards successful gene delivery in ...
    Determination of DNA Damage in the Retina Photoreceptors of Drosophila
    Authors:  Damulewicz Milena and Pyza Elzbieta, date: 02/05/2018, view: 88, Q&A: 0
    [Abstract] The retina is sensitive for light damages, because of direct light exposure, especially intense blue and UV light, which increase level of ROS and other toxic phototransduction products in photoreceptor cells. In our previous work (Damulewicz et al., 2017a and 2017b), we used 8-oxo-deoxyguanosine (8-OHdG) as a marker for oxidative stress ...
    Obtaining Acute Brain Slices
    Authors:  Thomas Papouin and Philip G. Haydon, date: 01/20/2018, view: 275, Q&A: 0
    [Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
    D-serine Measurements in Brain Slices or Other Tissue Explants
    Authors:  Thomas Papouin and Philip G. Haydon, date: 01/20/2018, view: 295, Q&A: 0
    [Abstract] D-serine is an atypical amino acid present in the mammalian body (most amino acids in the mammalian body are L-isomers) that is mostly known in neuroscience for its role as a co-agonist controlling the N-methyl D-aspartate receptor (NMDAR). D-serine levels are decreased in patients with schizophrenia and this is thought to mediate, at least in ...
    Mechanical Allodynia Assessment in a Murine Neuropathic Pain Model
    [Abstract] Experimental animal models are unique tools (i) to study pain transmission and pathophysiology of neuropathic pain, (ii) to identify novel molecular targets and (iii) to test the potential analgesic effect of specific molecules. The chronic constriction injury (CCI) model of neuropathic pain is the first model of post-traumatic painful peripheral ...
    Common Carotid Arteries Occlusion Surgery in Adult Rats as a Model of Chronic Cerebral Hypoperfusion
    Authors:  Dandan Cao, Yunfei Bai and Liang Li, date: 01/20/2018, view: 213, Q&A: 0
    [Abstract] Chronic cerebral hypoperfusion (CCH) is an important risk factor of vascular dementia (VaD) and Alzheimer’s disease (AD). Hypoxia/ischemia in the whole brain induced by CCH causes serious damage to brain structure and function, which can lead to cognitive impairment. Two-vessel occlusion (2-VO), also known as permanent, bilateral common carotid ...