Quantification of Thrips Damage Using Ilastik and ImageJ Fiji
[Abstract] Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available ...
Isolation of Nuclei in Tagged Cell Types (INTACT), RNA Extraction and Ribosomal RNA Degradation to Prepare Material for RNA-Seq
Authors: Mauricio A. Reynoso,
Germain C. Pauluzzi,
Sean Cabanlit,
Joel Velasco,
Jérémie Bazin,
Roger Deal,
Siobhan Brady,
Neelima Sinha,
Julia Bailey-Serres and
Kaisa Kajala,
date: 04/05/2018,
view: 667,
Q&A: 0
[Abstract] Gene expression is dynamically regulated on many levels, including chromatin accessibility and transcription. In order to study these nuclear regulatory events, we describe our method to purify nuclei with Isolation of Nuclei in TAgged Cell Types (INTACT). As nuclear RNA is low in polyadenylated transcripts and conventional pulldown methods would ...