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    Protocols in Current Issue
    Quantification of the Composition Dynamics of a Maize Root-associated Simplified Bacterial Community and Evaluation of Its Biological Control Effect
    Authors:  Ben Niu, Roberto Kolter and Rajneesh, date: 06/20/2018, view: 128, Q&A: 0
    Besides analyzing the composition and dynamics of microbial communities, plant microbiome research aims to understanding the mechanism of plant microbiota assembly and their biological functions. Here, we describe procedures to investigate the role ...
    Extraction and 16S rRNA Sequence Analysis of Microbiomes Associated with Rice Roots
    Authors:  Joseph Edwards, Christian Santos-Medellín and Venkatesan Sundaresan, date: 06/20/2018, view: 160, Q&A: 0
    Plant roots associate with a wide diversity of bacteria and archaea across the root-soil spectrum. The rhizosphere microbiota, the communities of microbes in the soil adjacent to the root, can contain up to 10 billion bacterial cells per gram of ...
    Increasing the Membrane Permeability of a Fern with DMSO
    Author:  Marcelo Garcés, date: 06/20/2018, view: 86, Q&A: 0
    Cell membrane prevents the entrance of extra molecules (e.g., transcription and translation inhibitors) into the cell. For studying the physiological effects of transcription and translation inhibitors on Hymenophyllum caudiculatum ...
    Analysis of Autophagic Activity Using ATG8 Lipidation Assay in Arabidopsis thaliana
    Authors:  Mengqian Luo, Xiaohong Zhuang and Rajneesh, date: 06/20/2018, view: 86, Q&A: 0
    As a fundamental metabolic pathway to degrade and recycle cellular cargos, autophagy is highly induced upon stress, starvation and senescence conditions in plants. A double-membrane structure named autophagosome will form during this process for ...
    Transient Expression Assay in NahG Arabidopsis Plants Using Agrobacterium tumefaciens
    Agrobacterium-mediated transient expression has greatly contributed to research in molecular plant biology but has low efficiency and inconsistency in Arabidopsis thaliana (Arabidopsis). Here, we describe a simple, ...
    Quantification of the Composition Dynamics of a Maize Root-associated Simplified Bacterial Community and Evaluation of Its Biological Control Effect
    Authors:  Ben Niu, Roberto Kolter and Rajneesh, date: 06/20/2018, view: 128, Q&A: 0
    [Abstract] Besides analyzing the composition and dynamics of microbial communities, plant microbiome research aims to understanding the mechanism of plant microbiota assembly and their biological functions. Here, we describe procedures to investigate the role of bacterial interspecies interactions in root microbiome assembly and the beneficial effects of the ...
    Extraction and 16S rRNA Sequence Analysis of Microbiomes Associated with Rice Roots
    Authors:  Joseph Edwards, Christian Santos-Medellín and Venkatesan Sundaresan, date: 06/20/2018, view: 160, Q&A: 0
    [Abstract] Plant roots associate with a wide diversity of bacteria and archaea across the root-soil spectrum. The rhizosphere microbiota, the communities of microbes in the soil adjacent to the root, can contain up to 10 billion bacterial cells per gram of soil (Raynaud and Nunan, 2014) and can play important roles for the fitness of the host plant. Subsets ...
    Increasing the Membrane Permeability of a Fern with DMSO
    Author:  Marcelo Garcés, date: 06/20/2018, view: 86, Q&A: 0
    [Abstract] Cell membrane prevents the entrance of extra molecules (e.g., transcription and translation inhibitors) into the cell. For studying the physiological effects of transcription and translation inhibitors on Hymenophyllum caudiculatum fronds, we incubate fronds with 0.1% DMSO to test if this increases cell membrane permeability ...
    Transient Expression Assay in NahG Arabidopsis Plants Using Agrobacterium tumefaciens
    [Abstract] Agrobacterium-mediated transient expression has greatly contributed to research in molecular plant biology but has low efficiency and inconsistency in Arabidopsis thaliana (Arabidopsis). Here, we describe a simple, efficient and fast protocol to make transient gene expression in NahG Arabidopsis plants using ...
    Analysis of Autophagic Activity Using ATG8 Lipidation Assay in Arabidopsis thaliana
    Authors:  Mengqian Luo, Xiaohong Zhuang and Rajneesh, date: 06/20/2018, view: 86, Q&A: 0
    [Abstract] As a fundamental metabolic pathway to degrade and recycle cellular cargos, autophagy is highly induced upon stress, starvation and senescence conditions in plants. A double-membrane structure named autophagosome will form during this process for cargo sequestration and delivery into the vacuole.

    A number of regulators have been ...
    Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
    Authors:  Agnès Pinel-Galzi, Eugénie Hébrard, Oumar Traoré, Drissa Silué and Laurence Albar, date: 06/05/2018, view: 228, Q&A: 0
    [Abstract] Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and ...
    Slow and Fast Desiccation of Single-cell Thick Fronds of Filmy Ferns
    Author:  Marcelo Garcés, date: 06/05/2018, view: 167, Q&A: 0
    [Abstract] Filmy ferns can desiccate and recover after rehydration to resume photosynthesis. Slow and fast desiccation rates were compared in filmy fern fronds to determine whether structural or physiological differences may occur between desiccation rates. Slow desiccation is considered to be more similar to natural conditions experienced by plants that ...
    Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana
    [Abstract] Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its’ inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to ...
    Rapid Screening and Evaluation of Maize Seedling Resistance to Stalk Rot Caused by Fusarium spp.
    Authors:  Yali Sun, Xinsen Ruan, Liang Ma, Fang Wang and Xiquan Gao, date: 05/20/2018, view: 364, Q&A: 0
    [Abstract] Corn stalk rot caused by Fusarium spp., a genus of soil-borne fungal pathogens, has become a major concern of maize production. This disease normally causes significant reduction of maize yield and quality worldwide. The field assay for identifying stalk rot resistance using adult plants is largely relying on large population, yet ...
    Infection Process Observation of Magnaporthe oryzae on Barley Leaves
    Author:  Xiao-Lin Chen, date: 05/05/2018, view: 429, Q&A: 0
    [Abstract] Rice blast and wheat blast caused by Magnaporthe oryzae is a serious threat to rice and wheat production. Appropriate methods for observing M. oryzae infection process are important for study of the fungal infection mechanisms, plant resistance reactions, and host-M. oryzae interactions. The rice leaf sheath is commonly ...