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    Protocols in Current Issue
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    Authors:  Alex Williams, Jurriaan Ton and Pierre Pétriacq, date: 05/20/2019, view: 319, Q&A: 0
    [Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...
    In vivo and in vitro 31P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in Hebeloma cylindrosporum in Response to Plant Roots Signals
    [Abstract] We used in vivo and in vitro phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus Hebeloma cylindrosporum in response to root signals originating from host (Pinus pinaster) or ...
    Quantification of the Composition Dynamics of a Maize Root-associated Simplified Bacterial Community and Evaluation of Its Biological Control Effect
    Authors:  Ben Niu and Roberto Kolter, date: 06/20/2018, view: 2467, Q&A: 0
    [Abstract] Besides analyzing the composition and dynamics of microbial communities, plant microbiome research aims to understanding the mechanism of plant microbiota assembly and their biological functions. Here, we describe procedures to investigate the role of bacterial interspecies interactions in root microbiome assembly and the beneficial effects of the ...
    Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light
    [Abstract] Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for ...
    Paper Roll Culture and Assessment of Maize Root Parameters
    Authors:  Adel H. Abdel-Ghani, Darlene L. Sanchez, Bharath Kumar and Thomas Lubberstedt, date: 09/20/2016, view: 6350, Q&A: 0
    [Abstract] Selection for genotypes with a vigorous root system could enhance the adaptation of maize under water and nutrient deficit soils. Although extensive genetic variation for root architecture has been reported (Kumar et al., 2012; Abdel-Ghani et al., 2014; Kumar et al., 2014; Pace et al., 2015), root traits have ...
    A Method to Analyze Local and Systemic Effects of Environmental Stimuli on Root Development in Plants
    Authors:  Neil E. Robbins II and José Dinneny, date: 09/05/2016, view: 5568, Q&A: 0
    [Abstract] Root development in vascular plants is innately tied to the environment. However, relatively little attention has been paid toward understanding the spatial scales at which the root perceives and responds to external stimuli. While some environmental signals elicit global responses that affect root system architecture, others may have more ...
    Assay of Arabinofuranosidase Activity in Maize Roots
    Authors:  Liudmila V. Kozlova, Polina V. Mikshina and Tatyana A. Gorshkova, date: 03/20/2016, view: 5086, Q&A: 0
    [Abstract] Root is a perfect model for studying the mechanisms of plant cell growth. Along the root length, several zones where cells are at different stages of development can be visualized (Figure 1). The dissection of the root on these zones allows the investigation of biochemical and genetic aspects of different growth steps. Maize primary root is much ...



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