Prokaryotes

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    Protocols in Current Issue
    Quantification of Chlorophyll as a Proxy for Biofilm Formation in the Cyanobacterium Synechococcus elongatus
    Authors:  Eleonora Sendersky, Ryan Simkovsky, Susan S. Golden and Rakefet Schwarz, date: 07/20/2017, view: 4250, Q&A: 0
    [Abstract] A self-suppression mechanism of biofilm development in the cyanobacterium Synechococcus elongatus PCC 7942 was recently reported. These studies required quantification of biofilms formed by mutants impaired in the biofilm-inhibitory process. Here we describe in detail the use of chlorophyll measurements as a proxy for biomass accumulation ...
    Quantitation of Cytochromes b559, b6, and f, and the Core Component of Photosystem I P700 in Cyanobacterial Cells
    Authors:  Motohide Aoki, Mikio Tsuzuki and Norihiro Sato, date: 11/05/2016, view: 3964, Q&A: 0
    [Abstract] Cytochrome (Cyt) b559, an important and essential core component of photosystem II in the photosynthetic electron transport system, is a heme-bridged heterodimer protein composed of an alpha subunit (PsbE) and a beta subunit (PsbE), and its reduced form has an absorption maximum in the α-band at 559 nm. The ...
    HPLC Analysis of Secreted Organic Acids
    Authors:  Fiona K. Davies, Sarah D’Adamo and Matthew C. Posewitz, date: 04/20/2016, view: 5940, Q&A: 0
    [Abstract] Under certain growth conditions some microorganisms secrete organic acids into the extracellular medium to relieve the accumulation of excess energy carriers, and/or to reduce toxic concentrations of organic acids. For example, a glycogen-deficient ∆glgC mutant of the cyanobacterium Synechococcus sp. PCC 7002 secretes pyruvate, ...
    Extraction and Quantification of Alkanes in Cyanobacteria
    [Abstract] Many species of cyanobacteria accumulate alkanes in their cells. It has been reported that these cyanobacteria accumulate mainly 15 long carbon chain alkane, pentadecane (C15H32), or/and 17 long chain alkane, heptadecane (C17H36). Here we describe a protocol of our laboratory for extraction and ...



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