Prokaryotes

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    Protocols in Current Issue
    Induction of Natural Competence in Genetically-modified Lactococcus lactis
    Authors:  Joyce Mulder, Michiel Wels, Oscar P. Kuipers, Michiel Kleerebezem and Peter A. Bron, date: 07/05/2018, view: 1541, Q&A: 0
    [Abstract] Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence by regulating the expression of the comX gene by using a nisin-inducible promoter in an L. ...
    Determination of Quinone Reductase Activity
    Authors:  Stefano Mancini and Marc Solioz, date: 09/05/2015, view: 4628, Q&A: 0
    [Abstract] We recently demonstrated the presence of a quinone detoxification pathway present in Firmicutes. It is based on two enzyme activities, namely a quinone reductase, YaiB, described here, and a hydroquinone dioxygenase, YaiA, described in a separate protocol. In Lactococcus lactis (L. lactis), these enzymes are encoded by the ...
    Determination of Hydroquinone Dioxygenase Activity
    Authors:  Stefano Mancini and Marc Solioz, date: 09/05/2015, view: 4317, Q&A: 0
    [Abstract] We recently demonstrated the presence of a quinone detoxification pathway present in Firmicutes. It is based on two enzyme activities, namely a hydroquinone dioxygenase, YaiA, described here, and a hydroquinone reductase, YaiB, described in a separate protocol. In Lactococcus lactis (L. lactis), these enzymes are encoded by the ...



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