Protocols in Current Issue
    Automatic Leaf Epidermis Assessment Using Fourier Descriptors in Texture Images
    [Abstract] The identification of plant species is not a trivial task, since it is carried out by experts and depends on the presence of fruits, flowers and leaves. However, fruits and flowers are not available throughout the year, while leaves are accessible most of the year. In order to assist the specialized work of species identification, methods of ...
    Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints
    Authors:  Telma E. Scarpeci, María I. Zanor and Estela M. Valle, date: 06/20/2017, view: 4966, Q&A: 2
    [Abstract] Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With ...
    Comprehensive Methods for Leaf Geometric Morphometric Analyses
    Authors:  Laura L. Klein and Harlan T. Svoboda, date: 05/05/2017, view: 7505, Q&A: 0
    [Abstract] Leaf morphometrics are used frequently by several disciplines, including taxonomists, systematists, developmental biologists, morphologists, agronomists, and plant breeders to name just a few. Leaf shape is highly variable and can be used for identifying species or genotypes, developmental patterning within and among individuals, assessing plant ...
    Differential and Simultaneous Visualization of Cells and Airspaces in Plant Leaves
    Authors:  Takashi Kawase, Shigeo S. Sugano, Tomoo Shimada and Ikuko Hara-Nishimura, date: 06/05/2016, view: 4529, Q&A: 0
    [Abstract] This protocol is to differentially and simultaneously visualize both cells and airspaces in intact leaves and to create 3D structures of cells and airspaces from confocal images using the software DSLT_Demo ( Leaves stained with Nile Red in ...
    Quantitative Image Analysis of Membrane Microdomains Labelled by Fluorescently Tagged Proteins in Arabidopsis thaliana and Nicotiana benthamiana
    Authors:  Iris K. Jarsch and Thomas Ott, date: 06/05/2015, view: 5465, Q&A: 0
    [Abstract] We have recently characterized co-existing membrane microdomains that are labeled by different proteins in living plant cells (Jarsch et al., 2014). For this approach we first created a digital fingerprint for each of the twenty marker proteins using quantitative image analysis. Here we recorded parameters such as domain size, density and ...

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