Prokaryotes

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    Protocols in Current Issue
    Protein Localization in the Cyanobacterium Anabaena sp. PCC7120 Using Immunofluorescence Labeling
    Authors:  Carla Trigo, Derly Andrade and Mónica Vásquez, date: 06/05/2017, view: 3674, Q&A: 0
    [Abstract] Techniques such as immunoflorescence are widely used to determine subcellular distribution of proteins. Here we report on a method to immunolocalize proteins in Anabaena sp. PCC7120 with fluorophore-conjugated antibodies by fluorescence microscopy. This method improves the permeabilization of cyanobacterial cells and minimizes the ...
    Fluorescence in situ Localization of Gene Expression Using a lacZ Reporter in the Heterocyst-forming Cyanobacterium Anabaena variabilis
    Authors:  Brenda S. Pratte and Teresa Thiel, date: 01/05/2017, view: 4092, Q&A: 0
    [Abstract] One of the most successful fluorescent proteins, used as a reporter of gene expression in many bacterial, plant and animals, is green fluorescent protein and its modified forms, which also function well in cyanobacteria. However, these fluorescent proteins do not allow rapid and economical quantitation of the reporter gene product, as does the ...
    Isolation of Heterocysts from Anabaena sp. PCC 7120
    Authors:  Maria Ermakova and Yagut Allahverdiyeva, date: 04/20/2015, view: 7963, Q&A: 0
    [Abstract] During combined nitrogen step-down, filaments of cyanobacterium Anabaena sp. PCC 7120 differentiate about 5-10% of vegetative photosynthetic cells into heterocysts, the specialized cells for N2 fixation (Walk, 1996). Heterocysts have a thick cell wall reducing permeation of O2 and consist of two additional layers ...



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