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    Protocols in Current Issue
    Measurement of Arabidopsis thaliana Plant Traits Using the PHENOPSIS Phenotyping Platform
    [Abstract] High-throughput phenotyping of plant traits is a powerful tool to further our understanding of plant growth and its underlying physiological, molecular, and genetic determinisms. This protocol describes the methodology of a standard phenotyping experiment in PHENOPSIS automated platform, which was engineered in INRA-LEPSE ( ...
    Determination of Boron Content Using a Simple and Rapid Miniaturized Curcumin Assay
    Authors:  Thotegowdanapalya C. Mohan and Alexandra M. E. Jones, date: 01/20/2018, view: 2798, Q&A: 0
    [Abstract] To determine boron quantity in soil, water and biological samples, several protocols are available. Colorimetric assays are the simplest and cheapest methods which can be used to determine boron concentration. However, published protocols do not give straightforward guidance for beginners to adopt these protocols for routine use in the laboratory. ...
    Quantifying the Capacity of Phloem Loading in Leaf Disks with [14C]Sucrose
    Authors:  Umesh P Yadav, Aswad S Khadilkar, Mearaj A Shaikh, Robert Turgeon and Brian G Ayre, date: 12/20/2017, view: 2419, Q&A: 0
    [Abstract] Phloem loading and transport of photoassimilate from photoautotrophic source leaves to heterotrophic sink organs are essential physiological processes that help the disparate organs of a plant function as a single, unified organism. We present three protocols we routinely use in combination with each other to assess (1) the relative rates of ...
    Assessing Rates of Long-distance Carbon Transport in Arabidopsis by Collecting Phloem Exudations into EDTA Solutions after Photosynthetic Labeling with [14C]CO2
    Authors:  Umesh P Yadav, Aswad S Khadilkar, Mearaj A Shaikh, Robert Turgeon and Brian G Ayre, date: 12/20/2017, view: 2414, Q&A: 0
    [Abstract] Phloem loading and transport of photoassimilate from photoautotrophic source leaves to heterotrophic sink organs are essential physiological processes that help the disparate organs of a plant function as a single, unified organism. We present three protocols we routinely use in combination with each other to assess (1) the relative rates of ...
    Pseudomonas syringae Flood-inoculation Method in Arabidopsis
    Authors:  Yasuhiro Ishiga, Takako Ishiga, Yuki Ichinose and Kirankumar S. Mysore, date: 01/20/2017, view: 5603, Q&A: 0
    [Abstract] Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inoculation method based on young Arabidopsis seedlings ...
    Hyaloperonospora arabidopsidis (Downy Mildew) Infection Assay in Arabidopsis
    Authors:  Shuta Asai, Ken Shirasu and Jonathan D. G. Jones, date: 10/20/2015, view: 8751, Q&A: 2
    [Abstract] Hyaloperonospora arabidopsidis (Hpa; formerly Peronospora parasitica or Hyaloperonospora parasitica) is an oomycete downy mildew pathogen of the model plant Arabidopsis. The pathosystem between Arabidopsis and Hpa has been extensively used to study host/pathogen co-evolution (Coates ...
    An Assay to Test Manganese Tolerance in Arabidopsis
    Authors:  Magdalena Migocka and Robert Biskup, date: 05/05/2015, view: 5146, Q&A: 0
    [Abstract] Manganese (Mn) is an essential nutrient required for the catalytic or regulatory function of several cellular enzymes. However, excessive Mn concentrations in plant tissues are toxic to plant cells as they negatively affect enzymatic activities, lead to oxidative stress and disturb the uptake and distribution of other essential mineral elements ...
    Pea Aphid Survival Assays on Arabidopsis thaliana
    Authors:  David C. Prince, Sam T. Mugford, Thomas R. Vincent and Saskia A. Hogenhout, date: 10/05/2014, view: 5926, Q&A: 0
    [Abstract] Aphids are phloem-feeding insects that successfully colonize specific host plant species. Aphid performance on a given plant is commonly measured by assessing fecundity of an aphid species that is adapted to the host. However, this approach may not reveal roles for plant genes in defense pathways that adapted aphids suppress. The host range of the ...
    Salinity Assay in Arabidopsis
    [Abstract] Salinity is an important environmental constraint to crop productivity in arid and semi-arid regions of the world. The evaluation of the responses to salinity of different Arabidopsis ecotypes or transgenic lines is important to identify and investigate the role of different key genes. These new characterized genes involved in the ...
    Determination of Water Use Efficiency for Arabidopsis thaliana
    Authors:  Weronika Wituszynska and Stanisław Karpiński, date: 02/05/2014, view: 13458, Q&A: 1
    [Abstract] Water use efficiency (WUE) is a quantitative measurement of how much biomass or yield is produced per amount of water used. It is an important physiological factor for agriculture, especially in areas with a limited accessibility of water. It is also crucial in a better understanding of drought tolerance and drought resistance. The most ...



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