Fish

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    Protocols in Current Issue
    How to Catch a Smurf? – Ageing and Beyond…
    In vivo Assessment of Intestinal Permeability in Multiple Model Organisms
    [Abstract] The Smurf Assay (SA) was initially developed in the model organism Drosophila melanogaster where a dramatic increase of intestinal permeability has been shown to occur during aging (Rera et al., 2011). We have since validated the protocol in multiple other model organisms (Dambroise et al., 2016) and have utilized the ...
    Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
    [Abstract] The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, ...
    Transplantation of Mesenchymal Cells Including the Blastema in Regenerating Zebrafish Fin
    Authors:  Eri Shibata, Kazunori Ando and Atsushi Kawakami, date: 01/20/2017, view: 5428, Q&A: 0
    [Abstract] Regeneration of fish fins and urodele limbs occurs via formation of the blastema, which is a mass of mesenchymal cells formed at the amputated site and is essential for regeneration. The blastema transplantation, a novel technique developed in our previous studies (Shibata et al., 2016; Yoshinari et al., 2012) is a useful ...
    Various Modes of Spinal Cord Injury to Study Regeneration in Adult Zebrafish
    Authors:  Subhra Prakash Hui and Sukla Ghosh, date: 12/05/2016, view: 7859, Q&A: 0
    [Abstract] Spinal cord injury (SCI) in mammals leads to failure of both sensory and motor functions, due to lack of axonal regrowth below the level of injury as well as inability to replace lost neural cells and to stimulate neurogenesis. In contrast, fish and amphibians are capable of regenerating a variety of their organs like limb/fin, jaw, heart and ...
    Thirty-Second Net Stressor Task in Adult Zebrafish
    Authors:  Steven Tran and Robert Gerlai, date: 03/05/2015, view: 6046, Q&A: 0
    [Abstract] Zebrafish have become a popular animal model for behavioral neuroscience (Gerlai, 2014). Recent studies have demonstrated that brief experimental handling prior to euthanizing animals can subsequently alter biological measures quantified post-mortem (e.g. cortisol levels) (Ramsay et al., 2009; Tran et al., 2014). Here we ...



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