Protocols in Current Issue
    Generation of BMEC Lines and in vitro BMEC-HSPC Co-culture Assays
    Authors:  Peipei Guo and Shahin Rafii, date: 11/05/2018, view: 1703, Q&A: 0
    [Abstract] Endothelial cells (ECs) sustain the self-renewal and regeneration of adult hematopoietic stem and progenitor cells (HSPCs) via deployment of EC-derived paracrine factors, termed as angiocrine factors. Generation of durable ex vivo vascular niche that maintains EC identity and preserves the angiocrine profile of organ of origin offers ...
    Isolation of Rodent Brain Vessels
    Authors:  Cristina Carvalho and Paula I. Moreira, date: 09/05/2017, view: 4350, Q&A: 0
    [Abstract] The prevalence of neurodegenerative diseases is increasing worldwide. Cerebrovascular disorders and/or conditions known to affect brain vasculature, such as diabetes, are well-known risk factors for neurodegenerative diseases. Thus, the evaluation of the brain vasculature is of great importance to better understand the mechanisms underlying brain ...
    Aorta Ring Assay
    Authors:  Jing Jin, Xinyang Hu, Ling Zhang and Jian'an Wang, date: 07/05/2016, view: 8264, Q&A: 1
    [Abstract] Angiogenesis is the nature and pathological process of blood vessel growth from pre-existing vascular buds. It plays an important role in cancer and cardiovascular disease. The aorta ring assay is an approach to study angiogenesis. In this experiment, we used the aorta of rat as the study material, cleaned the surrounding tissue of aorta and cut ...
    Transfection of Embryoid Bodies with miRNA Precursors to Induce Cardiac Differentiation
    Authors:  Federica Pisano and Massimiliano Gnecchi, date: 02/05/2016, view: 5283, Q&A: 1
    [Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the efficiency of cardiac ...
    Permanent Occlusion of the Left Anterior Coronary Artery in the Rat
    Authors:  Mélanie Bousquenaud, Pierre-Yves Marie and Yvan Devaux, date: 11/20/2015, view: 5153, Q&A: 0
    [Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological process is the main cause of cardiac evolution ...
    Rat Aortic Ring Model to Assay Angiogenesis ex vivo
    Authors:  Isabelle Ernens, Bénédicte Lenoir, Yvan Devaux and Daniel R. Wagner, date: 10/20/2015, view: 6428, Q&A: 0
    [Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These assays mainly use monolayers of ...
    Monocular Deprivation in Mice
    Authors:  Ya-tang Li, Xiao-lin Chou and Huizhong Whit Tao , date: 01/20/2014, view: 6606, Q&A: 0
    [Abstract] Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than three days (long term). Here we describe a ...
    Assay of Blood Brain Barrier and Placental Barrier Permeability
    [Abstract] Evans blue dye solution was used to observe the effect of a viral protein on two pre-defined barriers of the body i.e. the blood brain barrier (BBB) and the placental barrier (PB). This dye has strong affinity for serum albumin and does not cross these barriers under natural conditions. As all the dye gets bound to albumin, all the neural ...

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