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    Protocols in Current Issue
    Phototaxis Assay for Chlamydomonas reinhardtii
    Authors:  Noriko Ueki and Ken-ichi Wakabayashi, date: 06/20/2017, view: 5632, Q&A: 0
    [Abstract] Phototaxis is a behavior in which organisms move toward or away from the light source (positive or negative phototaxis, respectively). It is crucial for phototrophic microorganisms to inhabit under proper light conditions for phototaxis. The unicellular green alga Chlamydomonas reinhardtii rapidly changes its swimming direction upon light ...
    Cryopreservation Protocol for Chlamydomonas reinhardtii
    Authors:  Duanpeng Yang and Weiqi Li, date: 11/20/2016, view: 4613, Q&A: 0
    [Abstract] Cryopreservation is commonly used for storing viable cells, tissues, organs or organisms at ultralow temperatures, and usually involves immersion in liquid nitrogen at -196 °C. Here we provide a detailed cryopreservation protocol for C. reinhardtii based on Crutchfield’s work (Crutchfield et al., 1999), with minor changes (Yang ...
    Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography
    [Abstract] Cryo-electron tomography (CET) is a well-established technique for imaging cellular and molecular structures at sub-nanometer resolution. As the method is limited to samples that are thinner than 500 nm, suitable sample preparation is required to attain CET data from larger cell volumes. Recently, cryo-focused ion beam (cryo-FIB) milling of ...
    Ciliary and Flagellar Membrane Vesicle (Ectosome) Purification
    Author:  William Dentler, date: 06/20/2014, view: 6636, Q&A: 0
    [Abstract] Eukaryotic cilia/flagella are ideal organelles for the analysis of membrane trafficking, membrane assembly, and the functions of a variety of signal transduction molecules. Cilia are peninsular organelles and the membrane lipids, membrane proteins, and microtubular-associated components are selectively transported into cilia through the region ...
    Deflagellation and Regeneration in Chlamydomonas
    Author:  William Dentler, date: 06/20/2014, view: 9630, Q&A: 0
    [Abstract] Eukaryotic cilia/flagella are one of the only cellular structures that can be removed without injuring cells, can be highly purified for biochemical analysis, and, in many cells, can be completely reassembled within 90 minutes. Following amputation, the expression of many flagellar genes is up-regulated, and many are packaged and associated with ...



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