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    Protocols in Current Issue
    Arabidopsis Seed Germination Assay with Gibberellic Acid
    Authors:  Chunmei Zhong, Hao Xu, Siting Ye, Shengchun Zhang and Xiaojing Wang, date: 11/20/2016, view: 9874, Q&A: 2
    [Abstract] This assay analyzes Arabidopsis seed germination in response to gibberellic acid (GA). During seed imbibition, visible physiological changes allow precise determination of germination rate. This protocol utilizes a stereoscopic microscope to improve characterization of seed germination process.
    Cotyledon Wounding of Arabidopsis Seedlings
    Authors:  Debora Gasperini, Ivan F. Acosta and Edward E. Farmer, date: 01/20/2016, view: 6778, Q&A: 0
    [Abstract] Damage to plant organs through both biotic and abiotic injury is very common in nature. Arabidopsis thaliana 5-day-old (5-do) seedlings represent an excellent system in which to study plant responses to mechanical wounding, both at the site of the damage and in distal unharmed tissues. Seedlings of wild type, transgenic or mutant lines ...
    Imaging and Quantitative Analysis of Size and Distribution of Spherical Bodies, e.g. Embryonic Oil Bodies
    Authors:  Martine Miquel, Ghassen Trigui, Alain Trubuil and Bertrand Dubreucq, date: 01/05/2015, view: 5822, Q&A: 0
    [Abstract] Oil bodies (OBs) are seed-specific lipid storage organelles that allow the accumulation of neutral lipids that sustain plantlet development after the onset of germination. Using fluorescent dyes and confocal microscopy, we monitored the dynamics of OBs in living Arabidopsis (Arabidopsis thaliana) embryos at different stages of development ...
    Seed Storage Reserve Analysis
    Authors:  Harrie van Erp, Guillaume Menard and Peter J. Eastmond, date: 10/20/2014, view: 5785, Q&A: 0
    [Abstract] One of the major goals of plant research is to improve crop yield, by for instance increasing seed oil or protein content. Besides this, extensive research is done to change seed fatty acid (FA) composition in order to make vegetable oils more suitable for specific purposes. To determine the effect of genetic changes on seed FA composition, oil, ...
    Seed Coat Permeability Test: Tetrazolium Penetration Assay
    Authors:  Sollapura J. Vishwanath, Frédéric Domergue and Owen Rowland, date: 07/05/2014, view: 8995, Q&A: 1
    [Abstract] Seed coat permeability is important to study as it plays significant roles in seed dormancy, germination, and protection from pathogens. Here we describe a commonly used seed coat permeability test known as the tetrazolium penetration assay with a method to quantify the levels of permeability. Tetrazolium red is a cationic dye that is widely used ...
    Seed Germination and Viability Test in Tetrazolium (TZ) Assay
    Authors:  Pooja Verma and Manoj Majee, date: 09/05/2013, view: 43990, Q&A: 8
    [Abstract] Tetrazolium (TZ) assay is the fast evaluation for seed viability and alternative quick method for seed’s germinability (Porter et al., 1947; Wharton, 1955). All respiring tissues are capable of converting a colourless compound, TZ (2,3,5 triphenyl tetrazolium chloride) to a carmine red coloured water-insoluble formazan by hydrogen ...



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