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    Protocols in Current Issue
    Fabrication and Use of the Dual-Flow-RootChip for the Imaging of Arabidopsis Roots in Asymmetric Microenvironments
    [Abstract] This protocol provides a detailed description of how to fabricate and use the dual-flow-RootChip (dfRootChip), a novel microfluidic platform for investigating root nutrition, root-microbe interactions and signaling and development in controlled asymmetric conditions. The dfRootChip was developed primarily to investigate how plants roots interact ...
    Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple
    [Abstract] The Rapid Alkalinization Factor (RALF) is a plant hormone peptide that inhibits proton transport causing alkalinization of the extracellular media. To detect the alkalinization response elicited by RALF peptides in root cells, Arabidopsis seedlings are carefully transferred to a gel containing the pH-sensitive indicator bromocresol ...
    Analyses of Root-secreted Acid Phosphatase Activity in Arabidopsis
    Authors:  Liangsheng Wang and Dong Liu, date: 04/05/2017, view: 5202, Q&A: 0
    [Abstract] Induction and secretion of acid phosphatase (APase) is a universal adaptive response of higher plants to low-phosphate stress (Tran et al., 2010). The intracellular APases are likely involved in the remobilization and recycling of phosphate (Pi) from intracellular Pi reserves, whereas the extracellular or secreted APases are believed to ...
    In vitro CLE Peptide Bioactivity Assay on Plant Roots
    Authors:  Shiyan Chen and Xiaohong Wang, date: 12/20/2015, view: 5895, Q&A: 0
    [Abstract] Plant CLAVATA3/ESR (CLE)-related proteins play diverse roles in plant growth and development including regulating the development of root meristem. The mature functional forms of CLE peptides are typically 12-13 amino acids (aa) in length that are derived from the conserved C-termini of their precursor proteins. Genes encoding small secreted ...
    Two-photon Photoactivation to Measure Histone Exchange Dynamics in Plant Root Cells
    Authors:  Stefanie Rosa and Peter Shaw, date: 10/20/2015, view: 4519, Q&A: 0
    [Abstract] Chromatin-binding proteins play a crucial role in chromatin structure and gene expression. Direct binding of chromatin proteins both maintains and regulates transcriptional states. It is therefore important to study the binding properties of these proteins in vivo within the natural environment of the nucleus. Photobleaching, ...
    In vitro Colorimetric Method to Measure Plant Glutamate Dehydrogenase Enzyme Activity
    Authors:  Asier Sarasketa, Izargi Vega-Mas and Daniel Marino, date: 08/20/2015, view: 6380, Q&A: 1
    [Abstract] Glutamate dehydrogenase (GDH) is an NAD(H) dependent enzyme that catalyzes, in vitro, the reversible amination of glutamate. Here we describe how to determine spectrophotometrically GDH activity monitoring NADH evolution. This protocol is described here for Arabidopsis thaliana (A. thaliana) although it is also valid for ...
    Determination of Ferric Chelate Reductase Activity in the Arabidopsis thaliana Root
    Authors:  Emre Aksoy and Hisashi Koiwa, date: 08/05/2013, view: 10098, Q&A: 5
    [Abstract] Plants have developed two distinct mechanisms, i.e., strategy I (reduction strategy) and II (chelation strategy), to mobilize insoluble Fe(III) in the rhizosphere and transport it through the plasma membrane. Arabidopsis thaliana and other dicots rely on strategy I. In this strategy, the rhizosphere is first acidified by a ...



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