Mammalia

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    Protocols in Current Issue
    TRIPLE (Insulin, Glucagon and EGFP) Immunofluorescence Staining Protocol in Pancreas
    Authors:  Woogyun Choi, Randal J. Kaufman and Sung Hoon Back, date: 03/05/2014, view: 10739, Q&A: 0
    [Abstract] This protocol aims to introduce methods for immunostaining two endogenous proteins insulin and glucagon and one exogenous transgene driven EGFP in mouse pancreatic islet. The immunostaining results of insulin and glucagon indirectly tell functionality of pancreatic beta cells and alpha cells respectively. Furthermore, the protocol provides ...



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