Mammalia

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    Protocols in Current Issue
    Acute Cerebellar Slice Preparation Using a Tissue Chopper
    Authors:  Yubin Wang and Michel Baudry, date: 03/05/2019, view: 1246, Q&A: 0
    [Abstract] Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
    Eye Drops for Delivery of Bioactive Compounds and BrdU to Stimulate Proliferation and Label Mitotically Active Cells in the Adult Rodent Retina
    Authors:  Cindy L. Linn, Sarah E. Webster and Mark K. Webster, date: 11/05/2018, view: 1754, Q&A: 0
    [Abstract] Eye drop treatments are typically used to apply drugs to the anterior structures of the eye. Recently, however, studies have demonstrated that eye drops can reach the retina in the back of the eye if pharmacological agents are carried in appropriate vehicles. Here, we introduce an eye drop procedure to deliver a drug (PNU-282987), in combination ...
    Testing Effects of Chronic Chemogenetic Neuronal Stimulation on Energy Balance by Indirect Calorimetry
    Authors:  Sangho Yu and Heike Münzberg, date: 04/20/2018, view: 2474, Q&A: 0
    [Abstract] The fundamental of neuroscience is to connect the firing of neurons to physiological and behavioral outcomes. Chemogenetics enables researchers to control the activity of a genetically defined population of neurons in vivo through the expression of designer receptor exclusively activated by designer drug (DREADD) in specific neurons and ...
    Spared Nerve Injury Model of Neuropathic Pain in Mice
    Authors:  Joseph Cichon, Linlin Sun and Guang Yang, date: 03/20/2018, view: 6721, Q&A: 1
    [Abstract] Experimental models of peripheral nerve injury have been developed to study mechanisms of neuropathic pain in living animals. The spared nerve injury (SNI) model in rodents is a partial denervation model, in which the common peroneal and tibial nerves are injured, producing consistent and reproducible tactile hypersensitivity in the skin territory ...
    Obtaining Acute Brain Slices
    Authors:  Thomas Papouin and Philip G. Haydon, date: 01/20/2018, view: 7726, Q&A: 1
    [Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
    Optogenetic Mapping of Synaptic Connections in Mouse Brain Slices to Define the Functional Connectome of Identified Neuronal Populations
    Authors:  Susana Mingote, Nao Chuhma and Stephen Rayport, date: 01/05/2017, view: 5908, Q&A: 0
    [Abstract] Functional connectivity in a neural circuit is determined by the strength, incidence, and neurotransmitter nature of its connections (Chuhma, 2015). Using optogenetics the functional synaptic connections between an identified population of neurons and defined postsynaptic target neurons may be measured systematically in order to determine the ...
    Olfactory Recognition Memory Test in Mice
    Authors:  Stephanie A. Jacobs, Fengying Huang, Joe Z. Tsien and Wei Wei, date: 05/05/2016, view: 5728, Q&A: 0
    [Abstract] Olfactory memory is an ethologically relevant task that relies on a mouse’s innate ability to use olfaction to forage for food (Zou et al., 2015), and identify safe foods. Although many of the same brain areas involved in other forms of memory are also involved in olfactory memory, the mechanisms are different (Sanchez-Andrade et al. ...
    Social Recognition Memory Test in Rodents
    Authors:  Stephanie A. Jacobs, Fengying Huang, Joe Z. Tsien and Wei Wei, date: 05/05/2016, view: 9501, Q&A: 0
    [Abstract] Social recognition memory is essential for the establishment and maintenance of a rodent colony. Recognition memory is important for social hierarchy, mate and offspring recognition, and interspecies recognition. Interspecies recognition is vital for recognizing frequent visitors to the animal’s habitat and whether or not the visitors pose a ...
    Mouse Auditory Brainstem Response Testing
    Authors:  Omar Akil, A. E. Oursler, Kevin Fan and Lawrence R. Lustig, date: 03/20/2016, view: 10429, Q&A: 0
    [Abstract] The auditory brainstem response (ABR) test provides information about the inner ear (cochlea) and the central pathways for hearing. The ABR reflects the electrical responses of both the cochlear ganglion neurons and the nuclei of the central auditory pathway to sound stimulation (Zhou et al., 2006; Burkard et al., 2007). The ABR ...
    A Novel Task for Studying Memory of Occasional Events in Rats
    [Abstract] Episodic memory has been defined in humans as the conscious recollection of unique personal past experiences often occurring singly during daily life, including remembrance of what happened, where and when it happened (Tulving, 1972). Here, we propose and describe in details a novel protocol we recently used to test the ability of rats to form and ...



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