Measuring Breathing Patterns in Mice Using Whole-body Plethysmography
[Abstract] Respiratory dysfunction is among the main cause of severe and fatal pathologies worldwide. The use of effective experimental models and methodologies for the study of the pulmonary pathophysiology is necessary to prevent, control and cure these diseases. Plethysmography, a technique for the assessment of lung function, has been widely applied in ...
Acute Cerebellar Slice Preparation Using a Tissue Chopper
Authors: Yubin Wang and
Michel Baudry,
date: 03/05/2019,
view: 2530,
Q&A: 0
[Abstract] Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
Spared Nerve Injury Model of Neuropathic Pain in Mice
Authors: Joseph Cichon,
Linlin Sun and
Guang Yang,
date: 03/20/2018,
view: 9227,
Q&A: 1
[Abstract] Experimental models of peripheral nerve injury have been developed to study mechanisms of neuropathic pain in living animals. The spared nerve injury (SNI) model in rodents is a partial denervation model, in which the common peroneal and tibial nerves are injured, producing consistent and reproducible tactile hypersensitivity in the skin territory ...
Obtaining Acute Brain Slices
Authors: Thomas Papouin and
Philip G. Haydon,
date: 01/20/2018,
view: 11784,
Q&A: 1
[Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
Olfactory Recognition Memory Test in Mice
[Abstract] Olfactory memory is an ethologically relevant task that relies on a mouse’s innate ability to use olfaction to forage for food (Zou et al., 2015), and identify safe foods. Although many of the same brain areas involved in other forms of memory are also involved in olfactory memory, the mechanisms are different (Sanchez-Andrade et al. ...
Social Recognition Memory Test in Rodents
[Abstract] Social recognition memory is essential for the establishment and maintenance of a rodent colony. Recognition memory is important for social hierarchy, mate and offspring recognition, and interspecies recognition. Interspecies recognition is vital for recognizing frequent visitors to the animal’s habitat and whether or not the visitors pose a ...
Mouse Auditory Brainstem Response Testing
[Abstract] The auditory brainstem response (ABR) test provides information about the inner ear (cochlea) and the central pathways for hearing. The ABR reflects the electrical responses of both the cochlear ganglion neurons and the nuclei of the central auditory pathway to sound stimulation (Zhou et al., 2006; Burkard et al., 2007). The ABR ...