Fungi

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    Protocols in Current Issue
    Fluorescently Labelled Aerolysin (FLAER) Labelling of Candida albicans Cells
    Authors:  Sneh Lata Singh and Sneha Sudha Komath, date: 06/05/2017, view: 3824, Q&A: 0
    [Abstract] In this protocol we describe a nonradiolabelled labelling of GPI anchor in Candida albicans. The method uses a fluorescent probe to bind specifically to GPI anchors so that the level of GPI-anchored proteins at the cell surface can be measured. The labelling does not need permeabilization of cells and can be carried out in vivo.
    Detection of Hog1 Phosphorylation in Candida albicans in Response to an Antifungal Protein
    Authors:  Brigitte ME Hayes and Nicole L van der Weerden, date: 09/20/2014, view: 5652, Q&A: 0
    [Abstract] It is becoming increasingly apparent that stress signalling is important for tolerance of fungal species to antifungal chemicals and proteins. The high-osmolarity glycerol (HOG) pathway responds to a number of stressors including osmotic and oxidative stress. This protocol describes a method to detect activation of the Candida albicans ( ...
    Immunoblot Analysis of Histone H4 Acetylation and Histone H2A Phosphorylation in Candida albicans
    Authors:  Michael Tscherner and Karl Kuchler, date: 10/20/2013, view: 6099, Q&A: 0
    [Abstract] Posttranslational modifications of histones are required for different processes including transcription, replication and DNA damage repair. This protocol describes the preparation of a whole-cell extracts for the fungal pathogen Candida albicans. Furthermore, the extract is used to detect lysine acetylation of histone H4 as well as ...
    Candida albicans Mitochondrial Protein Import Assay
    Authors:  Victoria L Hewitt, Trevor Lithgow and Ana Traven, date: 02/20/2013, view: 6480, Q&A: 0
    [Abstract] We have established the use of Candida albicans as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular networks including cellular metabolism and the cell cycle. Unlike the model lab yeast Saccharomyces ...



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