Protocols in Current Issue
    Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff’s Reagent: A Histochemical Approach
    [Abstract] Lipid peroxidation is a physiological indicator of both biotic and abiotic stress responses, hence is often used as a biomarker to assess stress-induced cell damage or death. Here we demonstrate an easy, quick and cheap staining method to assess lipid peroxidation in plant tissues. In this methodology, Schiff’s reagent, is used to assay for ...
    Analysis of Total Se Content in Rice
    Authors:  Lianhe Zhang, Feiyan Yu, Kun Deng, Bin Hu and Chengcai Chu, date: 10/05/2014, view: 6018, Q&A: 0
    [Abstract] Total Se content in rice is normally low and it is difficult to determine it exactly because of Se volatilization and pollution during the digestion process. In this method, rice sample is digested thoroughly and Se volatilization is reduced greatly by designing a specific digestion tube, increasing digestion temperature by three steps, ...
    Determination of Nitrate Uptake and Accumulation Using 15N in Rice Seedlings
    Authors:  Zhong Tang and Guohua Xu, date: 08/20/2013, view: 6457, Q&A: 0
    [Abstract] Nitrogen-15 is a rare stable isotope of nitrogen. This isotope is often used in agricultural research. For example, Nitrogen-15 is used to trace mineral nitrogen compounds and translocate the nitrogen molecule in plants. This protocol is used to determine nitrate uptake and accumulation in rice seedlings by using Nitrogen-15.
    Histone Methyltransferase Assay in vitro
    Authors:  Pengfei Sui, Yu Yu and Aiwu Dong, date: 12/20/2012, view: 11737, Q&A: 0
    [Abstract] Histone methylation is an important epigenetic modification that plays important roles in plant development and growth. Histone methytransferases are the enzymes to establish histone methylation and here we describe a simple and effective protocol for detecting methyltransferase activity and specificity in vitro.

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