Protozoans

Categories

    Protocols in Current Issue
    Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
    Authors:  Amalia Papadaki and Haralabia Boleti, date: 10/05/2019, view: 235, Q&A: 0
    [Abstract] Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells. In bacteria and protozoan pathogens, acid ecto-phosphatases have been associated with the survival of intracellular pathogens within phagocytes through ...
    Protein Sample Preparation for Proteomic Analysis in Leishmania donovani
    Authors:  Alexandros Alexandratos and Despina Smirlis, date: 03/05/2014, view: 7426, Q&A: 0
    [Abstract] Leishmania is a genus of trypanosomatid protozoa and is the parasite responsible for the disease leishmaniasis. These protozoa, regulate their gene expression in an atypical way, compared to other higher eukaryotes. The regulation of gene expression is characterized by a predominance of post-transcriptional over pre-transcriptional ...
    Glycophosphosphingolipid (GSPL) Purification Protocol
    Author:  Tripti De, date: 12/05/2012, view: 7200, Q&A: 0
    [Abstract] Glycosylated ceramide phosphorylinositol are present in many species of fungi and mushrooms and bacteria and parasitic organisms like leishmania. These are usually membrane raft associated and are not easily extracted by conventional methodologies. This extraction method gives higher yield of the glycolipid. Glycosphingolipids are usually ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.