Prokaryotes

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    Protocols in Current Issue
    Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
    Authors:  Tomáš Zavřel, Maria A. Sinetova and Jan Červený, date: 05/05/2015, view: 17905, Q&A: 1
    [Abstract] This is a protocol for precise measurement of chlorophyll a and total carotenoid concentrations in cyanobacteria cells. Cellular chlorophyll concentration is one of the central physiological parameters, routinely followed in many research areas ranging from stress physiology to biotechnology. Carotenoids concentration is often related to ...
    Stable Transformation of Cyanobacterium Synechocystis sp.
    Author:  Reinhard K Proels, date: 11/05/2014, view: 8341, Q&A: 0
    [Abstract] Cyanobacteria are prokaryotes, which perform oxygenic photosynthesis. Among them, the unicellular cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis) is a well characterized model system for studies on oxygenic photosynthesis, light signal transduction etc. Moreover, Synechocystis is applied in ...
    Radiolabeling of Chlorophyll by [14C]Glutamic Acid in vivo and Relative Quantification of Labeled Chlorophyll by Using Thin Layer Chromatography (TLC)
    Authors:  Roman Sobotka, Luděk Kořený, Jana Kopečná and Miroslav Oborník, date: 08/20/2012, view: 6392, Q&A: 0
    [Abstract] This is an accurate method to assess the rate of chlorophyll biosynthesis in vivo in cyanobacteria. Given that labeled glutamate is used as the very early precursor of chlorophyll together with a short pulse of labeling (30 min), this method provides information about the metabolic flow through the whole chlorophyll biosynthetic pathway ...



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