Prokaryotes

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    Protocols in Current Issue
    Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila for Negative Stain Electron Microscopy Studies
    Authors:  Tomoko Kubori and Hiroki Nagai, date: 04/20/2017, view: 3718, Q&A: 0
    [Abstract] Legionella possesses a pivotal secretion machinery to deliver virulence factors to eukaryotic host cells. In this protocol, we describe the procedure for isolation of the native core complex of the Dot/Icm type IV secretion system from L. pneumophila aiming to perform biochemical and transmission electron microscopy analyses.
    ARP2/3 Phosphorylation Assay in the Presence of Recombinant Bacterial Effectors
    Authors:  Céline Michard, Lawrence L. LeClaire and Patricia Doublet, date: 04/05/2017, view: 4178, Q&A: 0
    [Abstract] The Actin-Related Protein 2/3 (ARP2/3) complex is an actin nucleator that generates a branched actin network in mammalian cells. In addition to binding nucleation promoting factors, LeClaire et al. demonstrated that its phosphorylation state is essential key for its activity (LeClaire et al., 2008). In cells, the ARP2/3 complex ...



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