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    Protocols in Current Issue
    Infection of Soybean Plants with the Insect Bacterial Symbiont Burkholderia gladioli and Evaluation of Plant Fitness
    Authors:  Paul Gaube, Martin Kaltenpoth and Laura V. Flórez, date: 12/20/2017, view: 2661, Q&A: 0
    [Abstract] To investigate the establishment and consequences of host-microbe interactions, it is important to develop controlled infection assays suitable for each system, as well as appropriate methods to evaluate successful infection and its associated effects. Here, we describe a procedure for bacterial inoculation of soybean plants, followed by the ...
    Simple Methods for Screening and Statistical Analysis of Leaf Epidermal Cells in Dicotyledonous Plants
    Authors:  Jian-Jun Tao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2016, view: 5905, Q&A: 0
    [Abstract] Leaf epidermal cell size and number are positively correlated to leaf area. Stomata are specialized epidermal cells vital for gas exchange and water transpiration. So, observation and statistical analysis of the leaf epidermal cells are valuable for the study of leaf development and response to environmental stimulus. The classical method is using ...
    Phakopsora pachyrhizi Infection Bioassay in Detached Soybean Transgenic Leaves for Candidate Gene Validation
    [Abstract] Soybean (Glycine max) is one of the most important crops in the world. Phakopsora pachyrhizi is a plant pathogenic basidiomycete fungus that infects soybean, causing Asian Soybean Rust (ASR) disease and affecting production. Here, we describe how to prepare the plant material and the uredospore suspension (from spores harvested ...
    Airbrush Infiltration Method for Pseudomonas syringae Infection Assays in Soybean
    Authors:  MB Shine, Da-Qi Fu and Aardra Kachroo, date: 03/20/2015, view: 6144, Q&A: 0
    [Abstract] We developed this protocol to assay the extent of proliferation of Pseudomonas syringae pv. glycinea in soybean leaves. This method specifically enables accurate pathogenesis assays of soybean plants at V2/V3 (2nd/3rd trifoliate) or higher stages of growth. The leaves of soybean plants at these growth stages ...



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