Mammalia

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    Protocols in Current Issue
    Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
    Authors:  Farzane Sivandzade, Aditya Bhalerao and Luca Cucullo, date: 01/05/2019, view: 4657, Q&A: 0
    [Abstract] In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the results across laboratories strongly depends upon following well validated and reliable protocols along with the appropriate controls. Herein, we provide a ...
    Generation of BMEC Lines and in vitro BMEC-HSPC Co-culture Assays
    Authors:  Peipei Guo and Shahin Rafii, date: 11/05/2018, view: 1897, Q&A: 0
    [Abstract] Endothelial cells (ECs) sustain the self-renewal and regeneration of adult hematopoietic stem and progenitor cells (HSPCs) via deployment of EC-derived paracrine factors, termed as angiocrine factors. Generation of durable ex vivo vascular niche that maintains EC identity and preserves the angiocrine profile of organ of origin offers ...
    Isolation of Rodent Brain Vessels
    Authors:  Cristina Carvalho and Paula I. Moreira, date: 09/05/2017, view: 4507, Q&A: 0
    [Abstract] The prevalence of neurodegenerative diseases is increasing worldwide. Cerebrovascular disorders and/or conditions known to affect brain vasculature, such as diabetes, are well-known risk factors for neurodegenerative diseases. Thus, the evaluation of the brain vasculature is of great importance to better understand the mechanisms underlying brain ...
    Functional ex-vivo Imaging of Arterial Cellular Recruitment and Lipid Extravasation
    [Abstract] The main purpose of this sophisticated and highly versatile method is to visualize and quantify structural vessel wall properties, cellular recruitment, and lipid/dextran extravasation under physiological conditions in living arteries. This will be of interest for a broad range of researchers within the field of inflammation, hypertension, ...
    Vascular Smooth Muscle Cell Isolation and Culture from Mouse Aorta
    Authors:  Callie S. Kwartler, Ping Zhou, Shao-Qing Kuang, Xue-Yan Duan, Limin Gong and Dianna M. Milewicz, date: 12/05/2016, view: 12115, Q&A: 0
    [Abstract] Vascular smooth muscle cells (SMC) in the ascending thoracic aorta arise from neural crest cells, whereas SMCs in the descending aorta are derived from the presomitic mesoderm. SMCs play important roles in cardiovascular development and aortic aneurysm formation. This protocol describes the detailed process for explanting ascending and descending ...
    Aorta Ring Assay
    Authors:  Jing Jin, Xinyang Hu, Ling Zhang and Jian'an Wang, date: 07/05/2016, view: 8498, Q&A: 1
    [Abstract] Angiogenesis is the nature and pathological process of blood vessel growth from pre-existing vascular buds. It plays an important role in cancer and cardiovascular disease. The aorta ring assay is an approach to study angiogenesis. In this experiment, we used the aorta of rat as the study material, cleaned the surrounding tissue of aorta and cut ...
    Preparation of Single Cell Suspensions from Mouse Aorta
    Authors:  Desheng Hu, Changjun Yin, Sarajo Mohanta, Christian Weber and Andreas J. R. Habenicht, date: 06/05/2016, view: 9893, Q&A: 0
    [Abstract] Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery tertiary lymphoid organs in the adventitia ...
    Aorta Atherosclerosis Lesion Analysis in Hyperlipidemic Mice
    Authors:  Sarajo Mohanta, Changjun Yin, Christian Weber, Desheng Hu and Andreas J. R. Habenicht, date: 06/05/2016, view: 11665, Q&A: 0
    [Abstract] Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE-/-) mice are used as experimental models to study human atherosclerosis. ApoE-/- mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various issues including ...
    Transfection of Embryoid Bodies with miRNA Precursors to Induce Cardiac Differentiation
    Authors:  Federica Pisano and Massimiliano Gnecchi, date: 02/05/2016, view: 5362, Q&A: 1
    [Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the efficiency of cardiac ...
    Permanent Occlusion of the Left Anterior Coronary Artery in the Rat
    Authors:  Mélanie Bousquenaud, Pierre-Yves Marie and Yvan Devaux, date: 11/20/2015, view: 5245, Q&A: 0
    [Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological process is the main cause of cardiac evolution ...



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