Protocols in Current Issue
    Method for Measuring Mucociliary Clearance and Cilia-generated Flow in Mice by ex vivo Imaging
    Authors:  Eric Song and Akiko Iwasaki, date: 03/20/2020, view: 379, Q&A: 0
    [Abstract] Ex vivo biophysical measurements provide valuable insights into understanding both physiological and pathogenic processes. One critical physiological mechanism that is regulated by these biophysical properties is cilia-generated flow that mediates mucociliary clearance, which is known to provide protection against foreign particles and ...
    Artificial Inhalation Protocol in Adult Mice
    Authors:  Thomas P. Eiting and Matt Wachowiak, date: 09/20/2018, view: 2280, Q&A: 0
    [Abstract] Research in the area of in vivo olfactory physiology benefits from having direct access to the nasal airways through which odorants can be presented. Ordinarily, the passage of odorants through the airways is controlled by respiratory rhythm. This fact makes it difficult to control the timing and strength of an olfactory stimulus, since ...
    Gastric Aspiration Models
    Authors:  Bruce A. Davidson and Ravi Alluri, date: 11/20/2013, view: 15259, Q&A: 0
    [Abstract] The procedures described below are for producing gastric aspiration pneumonitis in mice with alterations for rats and rabbits described parenthetically. We use 4 different injury vehicles delivered intratracheally to investigate the inflammatory responses to gastric aspiration:
    1) Normal saline (NS) as the injury vehicle control ...
    Measurement of Airway Responsiveness in the Anesthetized Mouse
    Authors:  François Daubeuf, Laurent Reber and Nelly Frossard, date: 04/05/2013, view: 9929, Q&A: 0
    [Abstract] Airway hyperresponsiveness to methacholine is an important characteristic of asthma. Many devices can be used to measure airway responsiveness in the mouse but it is well established that the invasiveness of a measurement technique is correlated with its precision and reproducibility. This protocol describes how to measure airway responses to ...
    Mast Cell Dependent Airway Hyperresponsiveness (AHR)
    Authors:  Yasutaka Motomura and Masato Kubo, date: 10/05/2012, view: 8652, Q&A: 0
    [Abstract] Asthma is a complex phenotype that involves multiple mechanisms, including adaptive and innate immunity as well as physiological and mechanical changes in the airways. In the models of asthma induced by sensitization and aerosolized allergen exposure in the absence of adjuvant, mast cells facilitate the development of in ammation and airway ...
    Mouse Transient Global Ischemia Two-Vessel Occlusion Model
    Authors:  Fabrizio Pontarelli, Dimitry Ofengeim, R. Suzanne Zukin and Elizabeth A. Jonas, date: 09/20/2012, view: 13546, Q&A: 0
    [Abstract] Transient global ischemia in rodents induces delayed death of hippocampal CA1 neurons, as well as in some hilar neurons of the dentate gyrus, medium aspiny neurons of the striatum, pyramidal neurons in neocortical layers II, V and VI, and Purkinje neurons of the cerebellum. In contrast to focal ischemia that mimics regional stroke in humans, this ...

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