Protocols in Current Issue
    Pancreatic Acinar Cell Preparation for Oxygen Consumption and Lactate Production Analysis
    Authors:  Jane A. Armstrong, Robert Sutton and David N. Criddle, date: 05/20/2020, view: 521, Q&A: 0
    [Abstract] Mitochondrial dysfunction is a principal feature of acute pancreatitis (AP) although the underlying mechanisms are still unclear. AP precipitants induce Ca2+-dependent formation of the mitochondrial permeability transition pore (MPTP) in pancreatic acinar cells (PACs), leading to ATP depletion and necrosis. Evaluations of mitochondrial ...
    Murine Pancreatic Islets Transplantation under the Kidney Capsule
    [Abstract] Type 1 diabetes (T1D) is an autoimmune disease caused by the lack of insulin-producing pancreatic beta cells leading to systemic hyperglycemia. Pancreatic islet transplantation is a valid therapeutic approach to restore insulin loss and to promote adequate glycemic control. Pancreatic islet transplantation in mice is an optimal preclinical model ...
    Isolation and Culture of the Islets of Langerhans from Mouse Pancreas
    Authors:  Kate L Graham, Stacey Fynch, Evan G Pappas, Christina Tan, Thomas WH Kay and Helen E Thomas, date: 06/20/2016, view: 16892, Q&A: 1
    [Abstract] The islets of Langerhans are clusters of endocrine cells located within the pancreas. Insulin-producing beta cells are the major cell type within islets, with glucagon-producing alpha cells and somatostatin-producing delta cells the other major cell types. The beta cells are the target of immune-mediated destruction in type 1 diabetes (Graham et ...
    TRIPLE (Insulin, Glucagon and EGFP) Immunofluorescence Staining Protocol in Pancreas
    Authors:  Woogyun Choi, Randal J. Kaufman and Sung Hoon Back, date: 03/05/2014, view: 11903, Q&A: 0
    [Abstract] This protocol aims to introduce methods for immunostaining two endogenous proteins insulin and glucagon and one exogenous transgene driven EGFP in mouse pancreatic islet. The immunostaining results of insulin and glucagon indirectly tell functionality of pancreatic beta cells and alpha cells respectively. Furthermore, the protocol provides ...
    Isolation, Culture and Differentiation of Primary Acinar Epithelial Explants from Adult Murine Pancreas
    Author:  Clara Lubeseder-Martellato, date: 07/05/2013, view: 8908, Q&A: 0
    [Abstract] The adult pancreas possesses an intrinsic developmental plasticity whereby acinar cells can convert into ductal structures under some pathological conditions. Acinar tissue can be isolated from murine pancreas and kept in three-dimensional collagen culture. Acinar to ductal metaplasia can be induced in primary acinar epithelial explants by ...

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