Prokaryotes

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    Protocols in Current Issue
    Determination of the in vitro Sporulation Frequency of Clostridium difficile
    Authors:  Adrianne N. Edwards and Shonna M. McBride, date: 02/05/2017, view: 4018, Q&A: 0
    [Abstract] The anaerobic, gastrointestinal pathogen, Clostridium difficile, persists within the environment and spreads from host-to-host via its infectious form, the spore. To effectively study spore formation, the physical differentiation of vegetative cells from spores is required to determine the proportion of spores within a population of C. ...
    EMS Mutagenesis of Clostridium difficile to Identify Strains with Germination-null Phenotypes
    Authors:  Michael B. Francis and Joseph A. Sorg, date: 12/20/2013, view: 7187, Q&A: 0
    [Abstract] Clostridium difficile is a Gram-positive, spore-forming, strict anaerobe and the leading cause of antibiotic-associated diarrhea (McFarland, 2008). Germination by C. difficile spores is the first step in pathogenesis. Thus, identifying mechanisms of C. difficile spore germination may lead to novel anti-germination ...



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