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    Protocols in Current Issue
    Isolation of Rice Stripe Virus Preparation from Viruliferous Small Brown Planthoppers and Mechanic Inoculation on Rice
    Authors:  Wan Zhao, Le Kang and Feng Cui, date: 11/05/2017, view: 3148, Q&A: 0
    [Abstract] Tenuiviruses can infect the plants of the family Poaceae, and cause serious loss of crops, particularly rice and maize, in South-Eastern Asian countries. Tenuiviruses usually depend on insect vectors for their transmission and cannot be transmitted between plants through wounds or abrasions. Rice stripe virus (RSV), a typical member of ...
    Rice Lamina Joint Inclination Assay
    Authors:  Hsing-Yi Li, Hsin-Mei Wang and Seonghoe Jang, date: 07/20/2017, view: 6580, Q&A: 0
    [Abstract] Brassinosteroids (BRs) promote rice lamina inclination. Recently, we showed that OsBUL1 knockout mutant rice (osbul1) is defective in brassinosteroid signaling (Jang et al., 2017). To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina ...
    Measurement of Stomatal Conductance in Rice
    Authors:  Yin Wang and Toshinori Kinoshita, date: 04/20/2017, view: 7169, Q&A: 0
    [Abstract] Stomatal conductance, the reciprocal of stomatal resistance, represents the gas exchange ability of stomata. Generally, the stomatal conductance is higher when stomata open wider, and vice versa. In this protocol, we describe how to measure stomatal conductance in rice using Li-6400 (Licor, USA).
    Inoculation of Rice with Different Pathogens: Sheath Blight (Rhizoctonia solani), Damping off Disease (Pythium graminicola) and Barley Powdery Mildew (Blumeria graminis f. sp. hordei)
    Authors:  Rhoda Delventhal, Marco Loehrer, Denise Weidenbach and Ulrich Schaffrath, date: 12/20/2016, view: 7902, Q&A: 0
    [Abstract] To prevent yield losses in plant cultivation due to plant pathogens, it is an important task to find new disease resistance mechanisms. Recently, Weidenbach et al. (2016) reported about the capacity of the rice gene OsJAC1 to enhance resistance in rice and barley against a broad spectrum of different pathogens. Here, we describe ...
    Bacterial Growth Inhibition Assay for Xanthomonas oryzae pv. oryzae or Escherichia coli K12 Grown together with Plant Leaf Extracts
    Authors:  Marco Loehrer, Rhoda Delventhal, Denise Weidenbach and Ulrich Schaffrath, date: 12/20/2016, view: 5313, Q&A: 0
    [Abstract] We performed a growth inhibition assay to test antibacterial compounds in leaf extracts from transgenic rice plants. The assay is based on over-night co-incubation of a defined concentration of colony forming units (cfu) of the respective bacteria together with aqueous extracts of ground leaf tissue.
    Detection of Reactive Oxygen Species in Oryza sativa L. (Rice)
    Authors:  Navdeep Kaur, Isha Sharma, Kamal Kirat and Pratap Kumar Pati, date: 12/20/2016, view: 10807, Q&A: 2
    [Abstract] Superoxide ions (O2-) and hydrogen peroxide (H2O2) are the reactive oxygen species (ROS) that play a significant role in regulation of many plant processes. The level of O2- ions is determined qualitatively using nitrobluetetrazolium (NBT) assay while the H2O2 is qualitatively ...
    Histochemical Staining of Silica Body in Rice Leaf Blades
    [Abstract] Silicon (Si) is a biologically important element for plants in the order Poales (Yamamoto et al., 2011; Kido et al., 2015). In rice, Si is mainly deposited in the motor cells and the cell walls of the leaf epidermis. However, the molecular basis of this overall process has not been elucidated. Thus, we propose a protocol for the ...
    Magnaporthe oryzae Inoculation of Rice Seedlings by Spraying with a Spore Suspension
    Authors:  Aya Akagi, Chang-Jie Jiang and Hiroshi Takatsuji, date: 06/05/2015, view: 9170, Q&A: 1
    [Abstract] Fungal blast caused by Magnaporthe oryzae (M. oryzae) leads to a serious yield loss of rice. Appropriate assessment of disease occurrence is necessary to investigate the nature of the disease and plant strategies to resist the disease. We describe our assay method of disease severity of M. oryzae on intact rice leaves ...
    RNA Editing Detection by Direct Sequencing
    [Abstract] RNA editing is a widespread post-transcriptional phenomenon through which primary RNA sequences are altered by nucleotide insertion/deletion or base conversion. It occurs in a variety of organisms and cooperates with alternative splicing in increasing both proteomic and transcriptomic complexity. We describe here a method allowing RNA editing ...
    An Efficient Procedure for Protoplast Isolation from Mesophyll Cells and Nuclear Fractionation in Rice
    [Abstract] Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in investigations of plant signal transduction pathways related to hormones, metabolites and environmental challenges. This protocol, adapted from ...



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