Protocols in Current Issue
    GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues
    Authors:  Manish Kumar Patel, Shubhashis Das and Jitendra Kumar Thakur, date: 09/20/2018, view: 3004, Q&A: 0
    [Abstract] Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and pharmacological industries due to their nutritional importance. In addition, FAs are considered as a promising ...
    Automatic Leaf Epidermis Assessment Using Fourier Descriptors in Texture Images
    [Abstract] The identification of plant species is not a trivial task, since it is carried out by experts and depends on the presence of fruits, flowers and leaves. However, fruits and flowers are not available throughout the year, while leaves are accessible most of the year. In order to assist the specialized work of species identification, methods of ...
    Quantification of Membrane Damage/Cell Death Using Evan’s Blue Staining Technique
    [Abstract] Membrane damage is a hallmark of both biotic and abiotic stress responses. The membrane determines the ability of a cell to sustain altered environmental conditions and hence can be used as a biomarker to assess stress-induced cell damage or death. We present an easy, quick, cost-effective, staining and spectrophotometric method to assess membrane ...
    Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints
    Authors:  Telma E. Scarpeci, María I. Zanor and Estela M. Valle, date: 06/20/2017, view: 4987, Q&A: 2
    [Abstract] Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With ...
    Photometric Assays for Chloroplast Movement Responses to Blue Light
    [Abstract] Assessment of chloroplast movements by measuring changes in leaf transmittance is discussed, with special reference to the conditions necessary for reliable estimation of blue light–activated chloroplast responses.
    Comprehensive Methods for Leaf Geometric Morphometric Analyses
    Authors:  Laura L. Klein and Harlan T. Svoboda, date: 05/05/2017, view: 7529, Q&A: 0
    [Abstract] Leaf morphometrics are used frequently by several disciplines, including taxonomists, systematists, developmental biologists, morphologists, agronomists, and plant breeders to name just a few. Leaf shape is highly variable and can be used for identifying species or genotypes, developmental patterning within and among individuals, assessing plant ...
    Miniature External Sapflow Gauges and the Heat Ratio Method for Quantifying Plant Water Loss
    Author:  Robert Paul Skelton, date: 02/05/2017, view: 4316, Q&A: 0
    [Abstract] External sapflow sensors are a useful tool in plant ecology and physiology for monitoring water movement within small stems or other small plant organs. These gauges make use of heat as a tracer of water movement through the stem and can be applied in both a laboratory and a field setting to generate data of relatively high temporal resolution. ...
    Differential and Simultaneous Visualization of Cells and Airspaces in Plant Leaves
    Authors:  Takashi Kawase, Shigeo S. Sugano, Tomoo Shimada and Ikuko Hara-Nishimura, date: 06/05/2016, view: 4543, Q&A: 0
    [Abstract] This protocol is to differentially and simultaneously visualize both cells and airspaces in intact leaves and to create 3D structures of cells and airspaces from confocal images using the software DSLT_Demo ( Leaves stained with Nile Red in ...
    Preparation of Mitotic and Meiotic Metaphase Chromosomes from Young Leaves and Flower Buds of Coccinia grandis
    [Abstract] Somatic chromosomes are usually studied from the root tip cells of the plants for cytological investigations. In dioecious plant species like Coccinia grandis, it is very difficult to get meristematic root tip cells from the mature plants of the respective sex forms. In this report, young leaves of the respective sexual phenotypes were ...
    In vitro Phosphorylation Assay of Putative Blue-light Receptor Phototropins Using Microsomal and Plasma-membrane Fractions Prepared from Vallisneria Leaves
    Authors:  Yuuki Sakai, Shin-ichiro Inoue and Shingo Takagi, date: 11/05/2015, view: 4975, Q&A: 0
    [Abstract] An aquatic angiosperm Vallisneria (Alismatales: Hydrocharitaceae) has been used as an excellent experimental material over a century to study the light regulation of dynamic intracellular movements including chloroplast redistribution and cytoplasmic streaming (Senn, 1908; Seitz, 1987; Takagi, 1997). However, understanding of the ...

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