Protocols in Current Issue
    Cytohistological Analyses of Mega-sporogenesis and Gametogenesis in Ovules of Limonium spp.
    Authors:  Ana S. Róis and Ana D. Caperta, date: 11/05/2016, view: 4912, Q&A: 0
    [Abstract] Limonium spp. are known to have sexual and apomixis (asexual reproduction through seeds) reproductive modes. Here, we present dissection protocol developed for ovules of Limonium spp. using differential interference contrast (DIC) microscopy. This protocol permits better handling of ovules and offers certain advantages over ...
    Ovule Clearing Method for Solanaceous Species
    Authors:  Audrey Loubert-Hudon and Daniel P. Matton, date: 11/05/2013, view: 8530, Q&A: 0
    [Abstract] Depending on the species, embryo sacs can be difficult to observe. Ovule clearing allows precise observation of whole tissues under Differential interference contrast (DIC) microscopy. The use of Methyl Salicylate as a clearing agent has proved to be particularly reliable for Solanaceous species.

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