Protocols in Current Issue
    Analysis of Monosaccharides from Arabidopsis Seed Mucilage and Whole Seeds Using HPAEC-PAD
    Authors:  Gillian H. Dean, Kresimir Sola, Faride Unda, Shawn D. Mansfield and George W. Haughn, date: 12/20/2019, view: 1350, Q&A: 0
    [Abstract] Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development. When mature seeds are hydrated, mucilage extrudes to form a gelatinous capsule around the seed. Determining the monosaccharide composition of ...
    Detection of Protein S-nitrosothiols (SNOs) in Plant Samples on Diaminofluorescein (DAF) Gels
    Authors:  Marta Rodríguez-Ruiz, Paulo T. Mioto, José M. Palma and Francisco J. Corpas , date: 09/20/2017, view: 4383, Q&A: 0
    [Abstract] In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (King et al., 2005) for plant samples. Briefly, ...
    Whole-seed Immunolabeling of Arabidopsis Mucilage Polysaccharides
    Author:  Cătălin Voiniciuc, date: 06/05/2017, view: 4448, Q&A: 0
    [Abstract] In addition to synthesizing and secreting copious amounts of pectic polymers (Young et al., 2008), Arabidopsis thaliana seed coat epidermal cells produce small amounts of cellulose and hemicelluloses typical of secondary cell walls (Voiniciuc et al., 2015c). These components are intricately linked and are released as a ...
    Arabidopsis Seed Germination Assay with Gibberellic Acid
    Authors:  Chunmei Zhong, Hao Xu, Siting Ye, Shengchun Zhang and Xiaojing Wang, date: 11/20/2016, view: 11947, Q&A: 2
    [Abstract] This assay analyzes Arabidopsis seed germination in response to gibberellic acid (GA). During seed imbibition, visible physiological changes allow precise determination of germination rate. This protocol utilizes a stereoscopic microscope to improve characterization of seed germination process.
    Metabolite Profiling of Mature Arabidopsis thaliana Seeds Using Gas Chromatography-Mass Spectrometry (GC-MS)
    Authors:  Hagai Cohen, Ifat Matityahu and Rachel Amir, date: 11/05/2016, view: 7507, Q&A: 0
    [Abstract] Metabolite profiling using gas chromatography-mass spectrometry (GC-MS) permits the annotation and quantification of a relatively wide variety of metabolites, covering a wide range of biochemical groups of metabolites. Lisec et al. (2006) established a method for GC-MS profiling in plants. Based on this protocol, we provide here a ...
    Analysis of Monosaccharides in Total Mucilage Extractable from Arabidopsis Seeds
    Authors:  Cătălin Voiniciuc and Markus Günl, date: 05/05/2016, view: 6753, Q&A: 0
    [Abstract] The Arabidopsis thaliana seed coat epidermis produces copious amounts of mucilage polysaccharides (Haughn and Western, 2012). Characterization of mucilage mutants has identified novel genes required for cell wall biosynthesis and modification (North et al., 2014). The biochemical analysis of seed mucilage is essential to evaluate ...
    Quantification of the Mucilage Detachment from Arabidopsis Seeds
    Author:  Cătălin Voiniciuc, date: 05/05/2016, view: 5292, Q&A: 0
    [Abstract] The Arabidopsis thaliana seed coat produces large amounts of cell wall polysaccharides, which swell out of the epidermal cells upon hydration of the mature dry seeds. While most mucilage polymers immediately diffuse in the surrounding solution, the remaining fraction tightly adheres to the seed, forming a dense gel-like capsule (Macquet ...
    Cotyledon Wounding of Arabidopsis Seedlings
    Authors:  Debora Gasperini, Ivan F. Acosta and Edward E. Farmer, date: 01/20/2016, view: 7863, Q&A: 0
    [Abstract] Damage to plant organs through both biotic and abiotic injury is very common in nature. Arabidopsis thaliana 5-day-old (5-do) seedlings represent an excellent system in which to study plant responses to mechanical wounding, both at the site of the damage and in distal unharmed tissues. Seedlings of wild type, transgenic or mutant lines ...
    Imaging and Quantitative Analysis of Size and Distribution of Spherical Bodies, e.g. Embryonic Oil Bodies
    Authors:  Martine Miquel, Ghassen Trigui, Alain Trubuil and Bertrand Dubreucq, date: 01/05/2015, view: 6599, Q&A: 0
    [Abstract] Oil bodies (OBs) are seed-specific lipid storage organelles that allow the accumulation of neutral lipids that sustain plantlet development after the onset of germination. Using fluorescent dyes and confocal microscopy, we monitored the dynamics of OBs in living Arabidopsis (Arabidopsis thaliana) embryos at different stages of development ...
    Vanillin Assay of Arabidopsis Seeds for Proanthocyanidins
    Authors:  Lijie Xuan, Zhong Wang and Lixi Jiang, date: 12/05/2014, view: 7671, Q&A: 0
    [Abstract] Proanthocyanidins (PAs) are colorless flavonoid polymers and deposit in Arabidopsis seed coat specifically. Oxidation of PAs gives rise to brown color of mature seeds. PA accumulation can be affected by a number of growth conditions, such as temperature and sun light. PAs, which are converted from anthocyanidins, can protect seeds from ...

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