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    Protocols in Current Issue
    Extraction and Assays of ADP-glucose Pyrophosphorylase, Soluble Starch Synthase and Granule Bound Starch Synthase from Wheat (Triticum aestivum L.) Grains
    Authors:  Konstantin Kulichikhin, Shalini Mukherjee and Belay T. Ayele, date: 09/20/2016, view: 7221, Q&A: 0
    [Abstract] Starch biosynthesis in plants involves a network of enzymes of which adenosine-5’-diphosphoglucose (ADP-glucose) pyrophosphorylase (AGPase, E.C. 2.7.7.27), and soluble and granule bound starch synthases (SSS and GBSS, E.C. 2.4.1.21) play central roles. Here, we outline the protocol for extraction and assay of these enzymes in developing grains of ...
    Analysis of Starch Synthase Activities in Wheat Grains using Native-PAGE
    Author:  Zhongyi Li, date: 01/20/2016, view: 6342, Q&A: 0
    [Abstract] Starch synthases are one class of key enzymes involving in the synthesis of cereal starch, which transfer glucose from ADP-glucose to the non-reducing end of pre-existing α-(1-4)-liked glucosyl chains of amylopectin. This protocol is highly reproducible for assaying activities for starch synthase I and IIIa in wheat and barley endosperm at ...
    In situ Digestion of Wheat Cell-wall Polysaccharides
    Authors:  Dušan Veličković and Hélène Rogniaux, date: 12/05/2014, view: 6707, Q&A: 2
    [Abstract] Cell walls of the wheat endosperm are mostly composed of arabinoxylans (AX) and mixed (1→3), (1→4)-β-glucans (BG) (Saulnier et al., 2012). Here, we present an optimized protocol to degrade enzymatically these cell-wall polysaccharides into oligosaccharides, directly from wheat grain cross sections. The main difficulty is to provide a ...
    Measuring Germination Percentage in Wheat
    Authors:  Harish Manmathan and Nora L.V. Lapitan, date: 08/20/2013, view: 12769, Q&A: 0
    [Abstract] Knowledge of the viability of seeds is a prerequisite for establishing the seeding rate in crop production and for germination-related trait evaluation in crop plants. This method explains a simple procedure to establish germination percentage in wheat seeds.



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