Protocols in Current Issue
    BMV Propagation, Extraction and Purification Using Chromatographic Methods
    [Abstract] Brome mosaic virus (BMV) is a well-known plant virus representing single-stranded RNA (ssRNA) positive-sense viruses. It has been widely used as a model in multiple studies concerning plant virus biology, epidemiology and the application of viral capsids in nanotechnology. Herein, we describe a method for BMV purification based on ion-exchange and ...
    Experimental Design to Determine Drought Stress Response and Early Leaf Senescence in Barley (Hordeum vulgare L.)
    Authors:  Gwendolin Wehner, Christiane Balko and Frank Ordon, date: 03/05/2016, view: 11005, Q&A: 1
    [Abstract] Premature leaf senescence induced by drought stress is a main factor for yield losses in barley. Research in drought stress tolerance has become more important as due to climate change the number of drought periods will increase and tolerance to drought stress has become a goal of high interest in barley breeding. However, reliable screening for ...
    Pyrosequencing Approach for SNP Genotyping in Plants Using a M13 Biotinylated Primer
    Authors:  Cristina Silvar, Dragan Perovic, Anna M Casas, Ernesto Igartua and Frank Ordon, date: 05/20/2015, view: 7302, Q&A: 0
    [Abstract] Single Nucleotide Polymorphisms (SNPs), which constitute single base-pair variations in the DNA sequence, are the most abundant molecular markers in plant and animal genomes. They are becoming the markers of choice for genotyping in all fields of molecular biology, as they are easily prone to automation and high throughput, for example through ...
    Fluorescence-based CAPS Multiplex Genotyping on Capillary Electrophoresis Systems
    Authors:  Jelena Perovic, Cristina Silvar, Dragan Perovic, Nils Stein and Frank Ordon, date: 05/20/2015, view: 6950, Q&A: 0
    [Abstract] Recent advances in next-generation sequencing techniques allow the detection of a large number of SNPs and their use in a high throughput manner. However, Cleaved Amplified Polymorphic Sequences (CAPSs) still play a significant role as complement to other high throughput methods for SNP genotyping. Therefore, new methods focusing on the ...
    Autoradiography of Pi Distribution in Barley Seedlings
    Authors:  Satomi Kanno, Yuko Kurita, Miwa Ohnishi and Tetsuro Mimura, date: 07/05/2014, view: 6472, Q&A: 0
    [Abstract] Phosphorus-32 and Phosphorus-33 are radioisotopes of phosphorus. These isotopes are used to trace ionic phosphorus and phosphorus compounds. This protocol is used to follow the movement of inorganic phosphate (PO43-) from a leaf tip to the rest of the plant.
    Modified Single-Cell Transient Gene Expression Assay in Barley Epidermal Cells
    Authors:  Shiwei Bai, Cheng Chang, Xinyun Han and Qian-Hua Shen, date: 05/05/2013, view: 7426, Q&A: 0
    [Abstract] Transient gene expression via biolistic particle delivery is a widely used technique for gene functional analysis in plants. In this protocol we describe a modified single-cell transient expression assay through transformation with a particle inflow gun of the model PDS-1000/He system (Bio-Rad). This assay was originally optimized for analyzing ...

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