Protocols in Current Issue
    Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly
    Authors:  Axel Himmelbach, Ines Walde, Martin Mascher and Nils Stein, date: 08/05/2018, view: 1754, Q&A: 0
    [Abstract] Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution and throughput of chromosome conformation capture (3C) (Dekker et al., 2002). The basic ...
    BMV Propagation, Extraction and Purification Using Chromatographic Methods
    [Abstract] Brome mosaic virus (BMV) is a well-known plant virus representing single-stranded RNA (ssRNA) positive-sense viruses. It has been widely used as a model in multiple studies concerning plant virus biology, epidemiology and the application of viral capsids in nanotechnology. Herein, we describe a method for BMV purification based on ion-exchange and ...
    Infection Process Observation of Magnaporthe oryzae on Barley Leaves
    Author:  Xiao-Lin Chen, date: 05/05/2018, view: 2446, Q&A: 0
    [Abstract] Rice blast and wheat blast caused by Magnaporthe oryzae is a serious threat to rice and wheat production. Appropriate methods for observing M. oryzae infection process are important for study of the fungal infection mechanisms, plant resistance reactions, and host-M. oryzae interactions. The rice leaf sheath is commonly ...
    Cytology and Microscopy: Immunolocalization of Covalently Modified Histone Marks on Barley Mitotic Chromosomes
    Authors:  Isabelle Colas, Katie Baker and Andrew J. Flavell, date: 06/20/2016, view: 4939, Q&A: 0
    [Abstract] Barley is a diploid inbreeding crop with a genome of 5 GB organized into seven chromosomes. The relatively low chromosome number and their large size make barley an excellent model for chromosome cytogenetic studies of large genome cereal crops. Chromatin can be defined as euchromatin or heterochromatin. Euchromatin is gene-rich, less condensed, ...
    Experimental Design to Determine Drought Stress Response and Early Leaf Senescence in Barley (Hordeum vulgare L.)
    Authors:  Gwendolin Wehner, Christiane Balko and Frank Ordon, date: 03/05/2016, view: 8379, Q&A: 0
    [Abstract] Premature leaf senescence induced by drought stress is a main factor for yield losses in barley. Research in drought stress tolerance has become more important as due to climate change the number of drought periods will increase and tolerance to drought stress has become a goal of high interest in barley breeding. However, reliable screening for ...
    Virus-induced Gene Silencing (VIGS) in Barley Seedling Leaves
    Authors:  Lokanadha R. Gunupuru, Shahin S. Ali, Fiona M. Doohan and Steven R. Scofield, date: 06/20/2015, view: 6818, Q&A: 0
    [Abstract] Virus induced gene silencing (VIGS) is one of the most potent reverse genetics technologies for gene functional characterisation. This method exploits a dsRNA-mediated antiviral defence mechanism in plants. Using this method allows researchers to generate rapid phenotypic data in a relatively rapid time frame as compared to the generation of ...
    Pyrosequencing Approach for SNP Genotyping in Plants Using a M13 Biotinylated Primer
    Authors:  Cristina Silvar, Dragan Perovic, Anna M Casas, Ernesto Igartua and Frank Ordon, date: 05/20/2015, view: 6209, Q&A: 0
    [Abstract] Single Nucleotide Polymorphisms (SNPs), which constitute single base-pair variations in the DNA sequence, are the most abundant molecular markers in plant and animal genomes. They are becoming the markers of choice for genotyping in all fields of molecular biology, as they are easily prone to automation and high throughput, for example through ...
    Fluorescence-based CAPS Multiplex Genotyping on Capillary Electrophoresis Systems
    Authors:  Jelena Perovic, Cristina Silvar, Dragan Perovic, Nils Stein and Frank Ordon, date: 05/20/2015, view: 5781, Q&A: 0
    [Abstract] Recent advances in next-generation sequencing techniques allow the detection of a large number of SNPs and their use in a high throughput manner. However, Cleaved Amplified Polymorphic Sequences (CAPSs) still play a significant role as complement to other high throughput methods for SNP genotyping. Therefore, new methods focusing on the ...
    Autoradiography of Pi Distribution in Barley Seedlings
    Authors:  Satomi Kanno, Yuko Kurita, Miwa Ohnishi and Tetsuro Mimura, date: 07/05/2014, view: 5342, Q&A: 0
    [Abstract] Phosphorus-32 and Phosphorus-33 are radioisotopes of phosphorus. These isotopes are used to trace ionic phosphorus and phosphorus compounds. This protocol is used to follow the movement of inorganic phosphate (PO43-) from a leaf tip to the rest of the plant.
    Extraction of Ions from Leaf Sections
    Authors:  Yuko Kurita, Satomi Kanno, Miwa Ohnishi and Tetsuro Mimura, date: 07/05/2014, view: 4412, Q&A: 0
    [Abstract] The concentration of ions in plant cells and tissues is an important factor to determine their functions and conditions. Here, we describe the method to extract ions from leaf sections for measurements with an ion chromatogram. This method is available for not only barley but also other plant species.

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