Protocols in Current Issue
    Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly
    Authors:  Axel Himmelbach, Ines Walde, Martin Mascher and Nils Stein, date: 08/05/2018, view: 1749, Q&A: 0
    [Abstract] Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution and throughput of chromosome conformation capture (3C) (Dekker et al., 2002). The basic ...
    Fusarium graminearum Inoculation on Wheat Head
    Authors:  Chanjing Feng, Huiquan Liu and Zhe Tang, date: 08/05/2018, view: 1744, Q&A: 0
    [Abstract] Fusarium graminearum, the major causal agent of Fusarium head blight (FHB), causes serious wheat yield losses and a threat to human and animal health. The main efforts to combat the disease are the research of pathogenesis mechanisms and breeding for disease resistance plants. The efficiency of these actions could be evaluated by ...
    Wheat Root-dip Inoculation with Fusarium graminearum and Assessment of Root Rot Disease Severity
    Authors:  Qing Wang and Sven Gottwald, date: 03/20/2017, view: 5158, Q&A: 0
    [Abstract] Fusarium graminearum is one of the most common and potent fungal pathogens of wheat (Triticum aestivum) and other cereals, known for causing devastating yield losses and mycotoxin contaminations of food and feed. The pathogen is mainly considered as a paradigm for the floral disease Fusarium head blight, while its ability to ...
    Assessment of Wheat Resistance to Fusarium graminearum by Automated Image Analysis of Detached Leaves Assay
    Authors:  Alexandre Perochon and Fiona M. Doohan, date: 12/20/2016, view: 5236, Q&A: 0
    [Abstract] Fusarium head blight (FHB) caused by Fusarium pathogens is a globally important cereal disease. To study Fusarium pathogenicity and host disease resistance, robust methods for disease assessment and quantification are needed. Here we describe the procedure of a detached leaves assay emphasizing the image analysis. The ...
    Extraction and Assays of ADP-glucose Pyrophosphorylase, Soluble Starch Synthase and Granule Bound Starch Synthase from Wheat (Triticum aestivum L.) Grains
    Authors:  Konstantin Kulichikhin, Shalini Mukherjee and Belay T. Ayele, date: 09/20/2016, view: 7226, Q&A: 0
    [Abstract] Starch biosynthesis in plants involves a network of enzymes of which adenosine-5’-diphosphoglucose (ADP-glucose) pyrophosphorylase (AGPase, E.C., and soluble and granule bound starch synthases (SSS and GBSS, E.C. play central roles. Here, we outline the protocol for extraction and assay of these enzymes in developing grains of ...
    Analysis of Starch Synthase Activities in Wheat Grains using Native-PAGE
    Author:  Zhongyi Li, date: 01/20/2016, view: 6345, Q&A: 0
    [Abstract] Starch synthases are one class of key enzymes involving in the synthesis of cereal starch, which transfer glucose from ADP-glucose to the non-reducing end of pre-existing α-(1-4)-liked glucosyl chains of amylopectin. This protocol is highly reproducible for assaying activities for starch synthase I and IIIa in wheat and barley endosperm at ...
    MIFE Technique-based Screening for Mesophyll K+ Retention for Crop Breeding for Salinity Tolerance
    Authors:  Honghong Wu, Lana Shabala, Meixue Zhou and Sergey Shabala, date: 05/05/2015, view: 5522, Q&A: 0
    [Abstract] Potassium is known as a rate-limiting factor for crop yield and plays an important role in plants response under abiotic stresses. Recently, cytosolic K+ retention ability in leaf mesophyll has emerged as an important component of plant salt tolerance mechanism (Wu et al., 2013; Wu et al., 2014; Wu et al., ...
    In situ Digestion of Wheat Cell-wall Polysaccharides
    Authors:  Dušan Veličković and Hélène Rogniaux, date: 12/05/2014, view: 6716, Q&A: 2
    [Abstract] Cell walls of the wheat endosperm are mostly composed of arabinoxylans (AX) and mixed (1→3), (1→4)-β-glucans (BG) (Saulnier et al., 2012). Here, we present an optimized protocol to degrade enzymatically these cell-wall polysaccharides into oligosaccharides, directly from wheat grain cross sections. The main difficulty is to provide a ...
    Rapid Induction of Water Stress in Wheat
    Authors:  Harish Manmathan and Nora L.V. Lapitan, date: 08/20/2013, view: 7297, Q&A: 0
    [Abstract] Traditional water stress evaluation studies in wheat are time consuming and can take up to several months to finish. A rapid phenotypic screening for water stress is important for accommodating time-bound water stress studies such as transient gene silencing studies in wheat. This method explains a procedure to induce water stress in young wheat ...
    Measuring Germination Percentage in Wheat
    Authors:  Harish Manmathan and Nora L.V. Lapitan, date: 08/20/2013, view: 12783, Q&A: 0
    [Abstract] Knowledge of the viability of seeds is a prerequisite for establishing the seeding rate in crop production and for germination-related trait evaluation in crop plants. This method explains a simple procedure to establish germination percentage in wheat seeds.

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