Prokaryotes

Categories

    Protocols in Current Issue
    Extraction and Analysis of Bacterial Teichoic Acids
    Authors:  Kelvin Kho and Timothy C. Meredith, date: 11/05/2018, view: 1504, Q&A: 0
    [Abstract] Teichoic acids (TA) are anionic polymers comprised of polyol phosphate repeat units that are abundant in the cell wall of Gram-positive bacteria. Both wall teichoic acid (WTA) and lipoteichoic acid (LTA) play important roles in regulating cell wall remodeling as well as conferring antibiotic resistance. To analyze TA, we describe a polyacrylamide ...
    In vitro Antigen-presentation Assay for Self- and Microbial-derived Antigens
    Author:  Laura Campisi, date: 06/05/2017, view: 7332, Q&A: 0
    [Abstract] Antigen presenting cells (APC) are able to process and present to T cells antigens from different origins. This mechanism is highly regulated, in particular by Patter Recognition Receptor (PRR) signals. Here, I detail a protocol designed to assess in vitro the capacity of APC to present antigens derived from bacteria, apoptotic and ...
    Adhesion and Invasion Assay Procedure Using Caco-2 Cells for Listeria monocytogenes
    Authors:  Swetha Reddy and Frank Austin, date: 05/05/2017, view: 6770, Q&A: 0
    [Abstract] Listeria monocytogenes is an important Gram-positive foodborne pathogen that is a particular problem in ready-to-eat food. It has an ability to survive in harsh conditions like refrigeration temperatures and high salt concentrations and is known to cross intestinal, placental and blood-brain barriers. Several cancerous cell lines like ...
    Purification of Bacterial RNA from Infected Macrophages
    Authors:  Lior Lobel, Nadejda Sigal, Anna Pasechnek and Anat A. Herskovits, date: 11/20/2015, view: 6099, Q&A: 0
    [Abstract] Studying the transcriptome of bacterial pathogens during infection is a very informative and effective tool for discovering genes that contribute to successful infection. However, isolating bacterial RNA from infected cells or tissues is a challenging process due to the much higher amounts of host RNA in the lysates of infected cells. We have ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.