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    Protocols in Current Issue
    Genome-wide Estimation of Evolutionary Distance and Phylogenetic Analysis of Homologous Genes
    Authors:  Meixia Zhao, Biao Zhang, Jianxin Ma and Damon Lisch, date: 12/05/2018, view: 1146, Q&A: 0
    [Abstract] Homologous genes, including paralogs and orthologs, are genes that share sequence homologies within or between different species. Homologous genes originate from a common origin through speciation, genetic duplication or horizontal gene transfer. Estimation of the sequence divergence of homologous genes help us to understand divergence time, which ...
    Infection of Soybean Plants with the Insect Bacterial Symbiont Burkholderia gladioli and Evaluation of Plant Fitness
    Authors:  Paul Gaube, Martin Kaltenpoth and Laura V. Flórez, date: 12/20/2017, view: 2649, Q&A: 0
    [Abstract] To investigate the establishment and consequences of host-microbe interactions, it is important to develop controlled infection assays suitable for each system, as well as appropriate methods to evaluate successful infection and its associated effects. Here, we describe a procedure for bacterial inoculation of soybean plants, followed by the ...
    Simple Methods for Screening and Statistical Analysis of Leaf Epidermal Cells in Dicotyledonous Plants
    Authors:  Jian-Jun Tao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2016, view: 5884, Q&A: 0
    [Abstract] Leaf epidermal cell size and number are positively correlated to leaf area. Stomata are specialized epidermal cells vital for gas exchange and water transpiration. So, observation and statistical analysis of the leaf epidermal cells are valuable for the study of leaf development and response to environmental stimulus. The classical method is using ...
    Agrobacterium rhizogenes-Based Transformation of Soybean Roots to Form Composite Plants
    Authors:  Benjamin F. Matthews and Reham M. Youssef, date: 01/20/2016, view: 5791, Q&A: 0
    [Abstract] Transgenic soybean roots of composite plants are a powerful tool to rapidly test the function of genes and activity of gene promoters. No tissue culture is needed, thus avoiding loss of valuable material due to contamination. This is a simple technique that requires less training and care than tissue culture techniques. Furthermore, it takes less ...
    Soybean Cyst Nematode, Heterodera glycines, Infection Assay Using Soybean Roots
    Authors:  Benjamin F. Matthews and Reham M. Youssef, date: 01/20/2016, view: 5811, Q&A: 0
    [Abstract] Soybean cyst nematode (SCN; Heterodera glycines), an obligate parasite of plants, is the most damaging pathogen of soybean, causing $469 to $818 million in soybean yield losses annually in the United States. However, there are no soybean cultivars available that are resistant to all SCN populations. Therefore, much research is being ...
    Phakopsora pachyrhizi Infection Bioassay in Detached Soybean Transgenic Leaves for Candidate Gene Validation
    [Abstract] Soybean (Glycine max) is one of the most important crops in the world. Phakopsora pachyrhizi is a plant pathogenic basidiomycete fungus that infects soybean, causing Asian Soybean Rust (ASR) disease and affecting production. Here, we describe how to prepare the plant material and the uredospore suspension (from spores harvested ...
    Airbrush Infiltration Method for Pseudomonas syringae Infection Assays in Soybean
    Authors:  MB Shine, Da-Qi Fu and Aardra Kachroo, date: 03/20/2015, view: 6132, Q&A: 0
    [Abstract] We developed this protocol to assay the extent of proliferation of Pseudomonas syringae pv. glycinea in soybean leaves. This method specifically enables accurate pathogenesis assays of soybean plants at V2/V3 (2nd/3rd trifoliate) or higher stages of growth. The leaves of soybean plants at these growth stages ...



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