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    Protocols in Current Issue
    Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae
    [Abstract] Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific ...
    Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer
    Authors:  Agnik Dasgupta, Andrew E. Jacob and Jeffrey D. Amack, date: 11/20/2018, view: 203, Q&A: 0
    [Abstract] A transient epithelial structure called the left-right organizer (LRO) establishes left-right asymmetry in vertebrate embryos. Developmental defects that alter LRO formation result in left-right patterning errors that often lead to congenital heart malformations. However, little is known about mechanisms that regulate individual cell behaviors ...
    Zebrafish Embryo Xenograft and Metastasis Assay
    Authors:  Ilkka Paatero, Sanni Alve, Silvia Gramolelli, Johanna Ivaska and Päivi M. Ojala, date: 09/20/2018, view: 659, Q&A: 0
    [Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...
    How to Catch a Smurf? – Ageing and Beyond…
    In vivo Assessment of Intestinal Permeability in Multiple Model Organisms
    [Abstract] The Smurf Assay (SA) was initially developed in the model organism Drosophila melanogaster where a dramatic increase of intestinal permeability has been shown to occur during aging (Rera et al., 2011). We have since validated the protocol in multiple other model organisms (Dambroise et al., 2016) and have utilized the ...
    zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
    [Abstract] In studies of brain function, it is essential to understand the underlying neuro-architecture. Very young zebrafish larvae are widely used for neuroarchitecture studies, due to their size and natural transparency. However, this model system has several limitations, due to the immaturity, high rates of development and limited behavioral repertoire ...
    Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
    [Abstract] The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, ...
    Measuring Behavioral Individuality in the Acoustic Startle Behavior in Zebrafish
    Authors:  Carlos Pantoja, Adam Hoagland, Elizabeth Carroll, David Schoppik and Ehud Y. Isacoff, date: 04/05/2017, view: 3610, Q&A: 0
    [Abstract] The objective of this protocol is to provide a detailed description for the construction and use of a behavioral apparatus, the zBox, for high-throughput behavioral measurements in larval zebrafish (Danio rerio). The zBox is used to measure behavior in multiple individuals simultaneously. Individual fish are housed in wells of multi-well ...
    Transplantation of Mesenchymal Cells Including the Blastema in Regenerating Zebrafish Fin
    Authors:  Eri Shibata, Kazunori Ando and Atsushi Kawakami, date: 01/20/2017, view: 3788, Q&A: 0
    [Abstract] Regeneration of fish fins and urodele limbs occurs via formation of the blastema, which is a mass of mesenchymal cells formed at the amputated site and is essential for regeneration. The blastema transplantation, a novel technique developed in our previous studies (Shibata et al., 2016; Yoshinari et al., 2012) is a useful ...
    Various Modes of Spinal Cord Injury to Study Regeneration in Adult Zebrafish
    Authors:  Subhra Prakash Hui and Sukla Ghosh, date: 12/05/2016, view: 5464, Q&A: 0
    [Abstract] Spinal cord injury (SCI) in mammals leads to failure of both sensory and motor functions, due to lack of axonal regrowth below the level of injury as well as inability to replace lost neural cells and to stimulate neurogenesis. In contrast, fish and amphibians are capable of regenerating a variety of their organs like limb/fin, jaw, heart and ...
    Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish
    Authors:  Toshihiro Kawasaki and Noriyoshi Sakai, date: 11/05/2016, view: 3291, Q&A: 0
    [Abstract] Allogeneic organ transplantation is a powerful tool for clinical and basic research studies. However, the graft is often rejected by the host organism. Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish. These zebrafish escaped rejection, which made it possible to successfully transplant fragments of an ...



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