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Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations

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Quantitative Determination of Ca²⁺-binding to Ca²⁺-sensor Proteins by Isothermal Titration Calorimetry

Authors: Seher Abbas and Karl-Wilhelm Koch, date: 04/05/2020, view: 687, Q&A: 0

[Abstract] Diverse and complex molecular recognitions are central elements of signal transduction cascades. The strength and nature of these interaction modes can be determined by different experimental approaches. Among those, Isothermal titration calorimetry (ITC) offers certain advantages by providing binding constants and thermodynamic parameters from ...

Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions

Authors: Faiza Basheer, Clifford Liongue and Alister C Ward, date: 03/05/2020, view: 796, Q&A: 0

[Abstract] The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host defense against any infection. The zebrafish is an ideal model to study the response of these cells ...

Retina Injury and Retina Tissue Preparation to Study Regeneration in Zebrafish

Authors: Poonam Sharma and Rajesh Ramachandran, date: 12/20/2019, view: 1298, Q&A: 0

[Abstract] Unlike mammals, primitive vertebrates have immense capability to regenerate almost all of their organs including the central nervous system. Among primitive organisms, zebrafish have been extensively used as a model system for regeneration studies. The retina is a part of the central nervous system and mammals lack the potential to repair any ...

Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans

Authors: Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova, date: 08/05/2019, view: 2906, Q&A: 0

[Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...

A Standardized Tank Design for the Light Dark Task in Zebrafish

Authors: Amanda Facciol, Steven Tran and Robert Gerlai, date: 07/20/2019, view: 1824, Q&A: 0

[Abstract] The light dark paradigm is a common behavioral test used to screen a variety of pharmacological agents, including anxiogenics and anxiolytics. Although most often used in rodents, the light dark task has recently been adapted for use in zebrafish. However, a number of inconsistent findings have been reported for this species. Some have found ...

Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos

Authors: Rachna Narayanan and Andrew C. Oates, date: 03/20/2019, view: 6091, Q&A: 0

[Abstract] In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by immunohistochemistry and coupled to ...

Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae

Authors: Zhiqiang Zeng, Juan C Lopez-Baez, Laura Lleras-Forero, Hannah Brunsdon, Cameron Wyatt, Witold Rybski, Nicholas D Hastie, Stefan Schulte-Merker and E Elizabeth Patton, date: 12/05/2018, view: 2179, Q&A: 0

[Abstract] Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific ...

Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer

Authors: Agnik Dasgupta, Andrew E. Jacob and Jeffrey D. Amack, date: 11/20/2018, view: 2495, Q&A: 0

[Abstract] A transient epithelial structure called the left-right organizer (LRO) establishes left-right asymmetry in vertebrate embryos. Developmental defects that alter LRO formation result in left-right patterning errors that often lead to congenital heart malformations. However, little is known about mechanisms that regulate individual cell behaviors ...

Zebrafish Embryo Xenograft and Metastasis Assay

Authors: Ilkka Paatero, Sanni Alve, Silvia Gramolelli, Johanna Ivaska and Päivi M. Ojala, date: 09/20/2018, view: 5511, Q&A: 1

[Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...

How to Catch a Smurf? – Ageing and Beyond…
In vivo Assessment of Intestinal Permeability in Multiple Model Organisms

Authors: Raquel R. Martins, Andrew W. McCracken, Mirre J. P. Simons, Catarina M. Henriques and Michael Rera, date: 02/05/2018, view: 6307, Q&A: 0

[Abstract] The Smurf Assay (SA) was initially developed in the model organism Drosophila melanogaster where a dramatic increase of intestinal permeability has been shown to occur during aging (Rera et al., 2011). We have since validated the protocol in multiple other model organisms (Dambroise et al., 2016) and have utilized the ...

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