Prokaryotes

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    Protocols in Current Issue
    Intracellular Invasion and Killing Assay to Investigate the Effects of Binge Alcohol Toxicity in Murine Alveolar Macrophages
    Authors:  Victor Jimenez Jr and Fernando P Monroy, date: 01/20/2019, view: 788, Q&A: 0
    [Abstract] Alcohol consumption has diverse and well-documented effects on the human immune system and its ability to defend against infective agents. While pulmonary related infections can occur in healthy humans, binge alcohol use is recognized as a major health risk factor (Nelson et al., 1991). Although binge alcohol consumption has been ...
    Bacteria-fungal Confrontation and Fungal Growth Prevention Assay
    [Abstract] There are some bacteria which can grow and multiply at the cost of living fungal biomass. They can potentially utilize fungi as a source of nutrients to forage over them. Such phenomenon is known as bacterial mycophagy, however, its mechanistic insights need to be explored to identify the molecules involved in mycophagy for potential utilization ...
    Infection of Soybean Plants with the Insect Bacterial Symbiont Burkholderia gladioli and Evaluation of Plant Fitness
    Authors:  Paul Gaube, Martin Kaltenpoth and Laura V. Flórez, date: 12/20/2017, view: 2651, Q&A: 0
    [Abstract] To investigate the establishment and consequences of host-microbe interactions, it is important to develop controlled infection assays suitable for each system, as well as appropriate methods to evaluate successful infection and its associated effects. Here, we describe a procedure for bacterial inoculation of soybean plants, followed by the ...
    Evaluation of Burkholderia cepacia Complex Bacteria Pathogenicity Using Caenorhabditis elegans
    Authors:  Pietro Tedesco, Elia Di Schiavi, Fortunato Palma Esposito and Donatella de Pascale, date: 10/20/2016, view: 3457, Q&A: 0
    [Abstract] This protocol describes two biological assays to evaluate pathogenicity of Burkholderia cepacia complex (Bcc) strains against the nematode Caenorhabditis elegans. Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing ...
    Testing the Effect of UV Radiation on the Survival of Burkholderia glumae
    Authors:  Hari Sharan Karki and Jong Hyun Ham, date: 03/05/2016, view: 5762, Q&A: 0
    [Abstract] Burkholderia glumae (B. glumae) is becoming a serious threat in the major rice producing areas of the world. It was reported that Burkholderia spp., including B. glumae, are adapted to a wide range of ecological niches. Different bacterial strains show different levels of UV tolerance which may be due to the ...
    Burkholderia glumae Competent Cells Preparation and Transformation
    Authors:  Hiromasa Saitoh and Ryohei Terauchi, date: 12/05/2013, view: 7120, Q&A: 1
    [Abstract] Bukholderia glumae is a gram-negative bacterium which causes grain rot, seedling rot and panicle blight in rice and bacterial wilt in many field crops. This bacterium has been reported from major rice growing regions around the world and is now considered as an emerging major pathogen of rice (Tsushima et al., 1996; Jeong et al. ...



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