Prokaryotes

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    Protocols in Current Issue
    Capturing Z-stacked Confocal Images of Living Bacteria Entering Hydathode Pores of Cauliflower
    Authors:  Aude Cerutti and Alain Jauneau, date: 10/20/2017, view: 4640, Q&A: 0
    [Abstract] The present protocol to visualize living bacteria at the pore level of cauliflower hydathodes is simple and trained users in confocal microscopy can execute it successfully. It can be easily adapted to capture images with other plant-microorganism interactions at the leaf surface and should be useful to obtain important information on pore and ...
    Xanthomonas oryzae pv. oryzae Inoculation and Growth Rate on Rice by Leaf Clipping Method
    Authors:  Yinggen Ke, Shugang Hui and Meng Yuan, date: 10/05/2017, view: 8593, Q&A: 0
    [Abstract] Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases and a major impediment to the increase of rice yield. Appropriate methods for inoculation of Xoo and disease scoring are necessary to investigate the nature of the disease and the mechanism of plant ...
    Xanthoferrin Siderophore Estimation from the Cell-free Culture Supernatants of Different Xanthomonas Strains by HPLC
    [Abstract] Xanthomonads can scavenge iron from the extracellular environment by secreting the siderophores, which are synthesized by the proteins encoded by xss (Xanthomonas siderophore synthesis) gene cluster. The siderophore production varies among xanthomonads in response to a limited supply of iron where Xanthomonas ...
    Extraction, Purification and Quantification of Diffusible Signal Factor Family Quorum-sensing Signal Molecules in Xanthomonas oryzae pv. oryzae
    Authors:  Lian Zhou, Xing-Yu Wang, Wei Zhang, Shuang Sun and Ya-Wen He, date: 03/20/2017, view: 5810, Q&A: 1
    [Abstract] Bacteria use quorum-sensing (QS) systems to monitor and regulate their population density. Bacterial QS involves small molecules that act as signals for bacterial communication. Many Gram-negative bacterial pathogens use a class of widely conserved molecules, called diffusible signal factor (DSF) family QS signals. The measurement of DSF family ...
    Isolation of Outer Membrane Vesicles from Phytopathogenic Xanthomonas campestris pv. campestris
    Authors:  Gideon Mordukhovich and Ofir Bahar, date: 03/05/2017, view: 5882, Q&A: 3
    [Abstract] Gram-negative bacteria naturally release outer membrane vesicles (OMVs) to the surrounding environment. OMVs contribute to multiple processes, such as cell-cell communication, delivery of enzymes and toxins, resistance to environmental stresses and pathogenesis. Little is known about OMVs produced by plant-pathogenic bacteria, and their ...
    Bioassay of Xanthomonas albilineans Attachment on Sugarcane Leaves
    Authors:  Imène Mensi, Jean-Heinrich Daugrois and Philippe Rott, date: 01/20/2017, view: 5575, Q&A: 0
    [Abstract] Sugarcane (interspecific hybrids of Saccharum species) is an economically important crop that provides 70% of raw table sugar production worldwide and contributes, in some countries, to bioethanol and electricity production. Leaf scald, caused by the bacterial plant pathogen Xanthomonas albilineans, is one of the major diseases ...
    Quantification of Ethylene Production in Tomato Leaves Infected by Xanthomonas euvesicatoria
    Authors:  Jung-Gun Kim, William Stork and Mary Beth Mudgett, date: 02/05/2016, view: 6344, Q&A: 0
    [Abstract] Ethylene is a gaseous plant hormone controlling fruit ripening, flower opening, leaf senescence as well as abscission, and disease symptom development. Ethylene plays a critical role in the bacterial pathogen Xanthomonas euvesicatoria (X. euvesicatoria)-elicited symptom development in tomato. This protocol describes the ...
    Assays to Assess Virulence of Xanthomonas axonopodis pv. manihotis on Cassava
    Authors:  Megan Cohn, Mikel Shybut, Douglas Dahlbeck and Brian Staskawicz, date: 07/05/2015, view: 5986, Q&A: 0
    [Abstract] Cassava (Manihot esculenta) is a root crop that provides calories for people living in more than 100 tropical and subtropical countries and serves as a raw material for processing into starch and biofuels as well as feed for livestock (Howeler et al., 2013). Xanthomonas axonopodis pv. manihotis (Xam), the causal ...
    Drug Sensitivity Assay of Xanthomonas citri subsp. citri Using REMA Plate Method
    Authors:  Isabel C. Silva and Henrique Ferreira, date: 08/20/2013, view: 7244, Q&A: 0
    [Abstract] Resazurin Microtiter Assay (REMA) is a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. Resazurin detects cell viability by converting from a nonfluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth (Palomino et al. ...



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