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    6-hydroxydopamine (6-OHDA) Oxidative Stress Assay for Observing Dopaminergic Neuron Loss in Caenorhabditis elegans
    Authors:  Sarah-Lena Offenburger and Anton Gartner, date: 09/20/2018, view: 35, Q&A: 0
    The nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many ...
    A Behavioral Assay to Examine the Effects of Kavalactones on Caenorhabditis elegans Neuromuscular Excitability
    Kavalactones are a class of lactone compounds found in Kava, a traditional beverage from the South Pacific Islands that is derived from the root of Piper methysticum. When consumed, these compounds produce sedative and anxiolytic effects, ...
    6-hydroxydopamine (6-OHDA) Oxidative Stress Assay for Observing Dopaminergic Neuron Loss in Caenorhabditis elegans
    Authors:  Sarah-Lena Offenburger and Anton Gartner, date: 09/20/2018, view: 35, Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many neuronal signaling components, such as those mediating dopaminergic neurotransmission, are preserved ...
    A Behavioral Assay to Examine the Effects of Kavalactones on Caenorhabditis elegans Neuromuscular Excitability
    [Abstract] Kavalactones are a class of lactone compounds found in Kava, a traditional beverage from the South Pacific Islands that is derived from the root of Piper methysticum. When consumed, these compounds produce sedative and anxiolytic effects, suggesting their potent actions on the nervous system. Here, we provide a protocol to examine the ...
    Pentylenetetrazole (PTZ)-induced Convulsion Assay to Determine GABAergic Defects in Caenorhabditis elegans
    Authors:  Shruti Thapliyal and Kavita Babu, date: 09/05/2018, view: 113, Q&A: 0
    [Abstract] Pentylenetetrazole (PTZ) is a GABAA receptor antagonist and is used to monitor presynaptic defects in the release of the inhibitory neurotransmitter GABA. PTZ is a competitive inhibitor of GABA, and prevents binding of GABA on the GABAA receptors present on the surface of muscle. In the absence of GABA binding, the excitatory ...
    Assessment of Caenorhabditis elegans Competitive Fitness in the Presence of a Bacterial Parasite
    Authors:  McKenna. J. Penley and Levi T. Morran, date: 08/20/2018, view: 265, Q&A: 0
    [Abstract] Accurate measurements of an organism’s fitness are crucial for measuring evolutionary change. Methods of fitness measurement are most accurate when incorporating an individual’s survival and fecundity, as well as accounting for any ecological interactions or environmental effects experienced by the organism. Here, we describe a protocol for ...
    FRAP: A Powerful Method to Evaluate Membrane Fluidity in Caenorhabditis elegans
    Authors:  Ranjan Devkota and Marc Pilon, date: 07/05/2018, view: 588, Q&A: 0
    [Abstract] FRAP (Fluorescence Recovery After Photobleaching) is probably the most direct method to investigate the dynamics of molecules in living cells. Here, we describe FRAP to quantify membrane fluidity in C. elegans. Using FRAP, we have shown that cold, glucose and exogenous saturated fatty acids can decrease the fluidity of cellular membranes ...
    Measuring Spatiotemporal Dynamics of Odor Gradient for Small Animals by Gas Chromatography
    Authors:  Akiko Yamazoe-Umemoto, Yuishi Iwasaki and Koutarou D. Kimura, date: 04/05/2018, view: 1260, Q&A: 0
    [Abstract] Odor is the most fundamental chemical stimulus that delivers information regarding food, mating partners, enemies, and danger in the surrounding environment. Research on odor response in animals is widespread, although studies on experimental systems in which the gradient of odor concentration is quantitatively measured has been quite limited. ...
    Measurement of ROS in Caenorhabditis elegans Using a Reduced Form of Fluorescein
    Authors:  Shaarika Sarasija and Kenneth R. Norman, date: 04/05/2018, view: 1019, Q&A: 0
    [Abstract] Oxidative stress is implicated in the pathogenesis of various neurodegenerative diseases, including Alzheimer’s disease. Oxidative stress is a result of a disruption of the equilibrium between antioxidants and oxidants, in favor of oxidants. Since mitochondria are major sites of production and reduction of reactive oxygen species (ROS), ...
    Analysis of Mitochondrial Structure in the Body Wall Muscle of Caenorhabditis elegans
    Authors:  Shaarika Sarasija and Kenneth R. Norman, date: 04/05/2018, view: 1172, Q&A: 0
    [Abstract] Mitochondrial function is altered in various pathologies, highlighting the crucial role mitochondria plays in maintaining cellular homeostasis. Mitochondrial structure undergoes constant fission and fusion in response to changing cellular environment. Due to this, analyzing mitochondrial structure could provide insight into the physiological state ...
    In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.
    [Abstract] The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the ...
    Measurement of Intracellular ROS in Caenorhabditis elegans Using 2’,7’-Dichlorodihydrofluorescein Diacetate
    Authors:  Dong Suk Yoon, Myon-Hee Lee and Dong Seok Cha, date: 03/20/2018, view: 1745, Q&A: 0
    [Abstract] Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, ...