Neuroscience

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    Protocols in Current Issue
    Protocol for Measuring Free (Low-stress) Exploration in Rats
    Authors:  Wojciech Pisula and Klaudia Modlinska, date: 01/20/2020, view: 823, Q&A: 0
    [Abstract] Research on exploratory behavior plays a key part in behavioral science. Studying exploratory behavior of laboratory rodents may provide important data about many developmental and neurobiological processes occurring in animal ontogenesis. The proposed protocol for measuring the free (low-stress) exploration behavior in rats is straightforward, ...
    Assessing Rough-and-tumble Play Behavior in Juvenile Rats
    Authors:  Jonathan W. VanRyzin, Ashley E. Marquardt and Margaret M. McCarthy, date: 01/05/2020, view: 1032, Q&A: 0
    [Abstract] Play is a complex social behavior that is highly conserved across mammals. In most species, males engage in more frequent and vigorous play as juveniles than females, which reflects subtle yet impactful sex differences in brain circuitry and development. In this protocol, we describe a behavioral testing paradigm to assess social play in male and ...
    Use of the Vsoc-maze to Study Sociability and Preference for Social Novelty in Rodents
    [Abstract] Studying social behavior in mouse models empowers the understanding of the neurobiological mechanisms involved, which are affected in neuropsychiatric disorders, allowing the evaluation of therapeutic strategies. Behavioral methods available are time-consuming and reducing the length of behavioral sessions may render more manageable experiments ...
    Behavioral Evaluation of Seeking and Preference of Alcohol in Mice Subjected to Stress
    Authors:  Ana Canseco-Alba, Norman Schanz, Hiroki Ishiguro, Qing-Rong Liu and Emmanuel S. Onaivi, date: 10/20/2018, view: 2346, Q&A: 0
    [Abstract] The alcohol preference model is one of the most widely used animal models relevant to alcoholism. Stressors increase alcohol consumption. Here we present a protocol for a rapid and useful tool to test alcohol preference and stress-induced alcohol consumption in mice. In this model, animals are given two bottles, one with a diluted solution of ...
    Shock-probe Defensive Burying Test to Measure Active versus Passive Coping Style in Response to an Aversive Stimulus in Rats
    Authors:  Elizabeth A. Fucich and David A. Morilak, date: 09/05/2018, view: 2402, Q&A: 0
    [Abstract] Maladaptive avoidance behaviors are seen in many stress-related psychiatric illnesses. Patients with these illnesses favor passive, avoidant coping strategies rather than adaptive, active coping strategies. Preclinically, coping strategy can be measured in rats using the shock-probe defensive burying test, wherein rats receive a shock from an ...
    Testing Effects of Chronic Chemogenetic Neuronal Stimulation on Energy Balance by Indirect Calorimetry
    Authors:  Sangho Yu and Heike Münzberg, date: 04/20/2018, view: 2987, Q&A: 0
    [Abstract] The fundamental of neuroscience is to connect the firing of neurons to physiological and behavioral outcomes. Chemogenetics enables researchers to control the activity of a genetically defined population of neurons in vivo through the expression of designer receptor exclusively activated by designer drug (DREADD) in specific neurons and ...
    Mechanical Allodynia Assessment in a Murine Neuropathic Pain Model
    [Abstract] Experimental animal models are unique tools (i) to study pain transmission and pathophysiology of neuropathic pain, (ii) to identify novel molecular targets and (iii) to test the potential analgesic effect of specific molecules. The chronic constriction injury (CCI) model of neuropathic pain is the first model of post-traumatic painful peripheral ...
    Formalin Murine Model of Pain
    Authors:  Marc López-Cano, Víctor Fernández-Dueñas, Amadeu Llebaria and Francisco Ciruela, date: 12/05/2017, view: 5709, Q&A: 0
    [Abstract] Pain research is mostly based on experimental assays that use animal models, which may allow deciphering the physiopathology of this condition and to propel drug discovery. The formalin nociception test is considered one of the most predictive approaches to study acute pain in rodents. This test permits monitoring pain-related responses (i.e. ...
    Touchscreen-based Visual Discrimination and Reversal Tasks for Mice to Test Cognitive Flexibility
    Authors:  Karly M. Turner, Christopher G. Simpson and Thomas H. J. Burne, date: 10/20/2017, view: 5878, Q&A: 0
    [Abstract] Reversal learning can be used to examine deficits in cognitive flexibility, which have been linked to a number of neuropsychiatric disorders including schizophrenia and addiction. However, methods of examining reversal learning have varied substantially between species. Touchscreen technology has allowed researchers to explore cognitive deficits ...
    Stereotaxic Adeno-associated Virus Injection and Cannula Implantation in the Dorsal Raphe Nucleus of Mice
    Authors:  Patrícia A. Correia, Sara Matias and Zachary F. Mainen, date: 09/20/2017, view: 9889, Q&A: 0
    [Abstract] Optogenetic methods are now widespread in neuroscience research. Here we present a detailed surgical procedure to inject adeno-associated viruses and implant optic fiber cannulas in the dorsal raphe nucleus (DRN) of living mice. Combined with transgenic mouse lines, this protocol allows specific targeting of serotonin-producing neurons in the ...



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