Plant Science

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    Protocols in Current Issue
    Heterologous Production and Anaerobic Purification of His- and StrepII-tagged Recombinant Proteins
    Author:  Jens Noth, date: 09/05/2013, view: 13114, Q&A: 0
    [Abstract] This protocol describes the heterologous expression and purification of proteins related to anoxic hydrogen production of Chlamydomonas reinhardtii (Noth et al., 2013). For this, the bacterial expression hosts Escherichia coli BL21 (DE3) ΔiscR (Akhtar MK et al., 2008) and Clostridium acetobutylicum ...



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