Protocols in Current Issue
    Estimation of the Minimum Number of Replication Origins Per Chromosome in any Organism
    Author:  Marcelo S. da Silva, date: 10/20/2020, view: 1640, Q&A: 0
    [Abstract] Eukaryote nuclear genomes predominantly replicate through multiple replication origins. The number of replication origins activated per chromosome during the S-phase duration may vary according to many factors, but the predominant one is replication stress. Several studies have applied different approaches to estimate the number and map the ...
    Introduction and Sequencing of Patient-isolated HBV RT Sequences into the HBV 1.2-mer Replicon
    Authors:  Sung Hyun Ahn, Yong Kwang Park and Kyun-Hwan Kim, date: 04/20/2015, view: 7581, Q&A: 0
    [Abstract] Antiviral agents for the suppression of hepatitis B virus (HBV) have been used for treating chronic hepatitis B. However, the emergence of drug-resistant HBV is still a major problem for antiviral treatment. To identify and characterize the drug-resistant HBV, the construction of HBV replicon and in vitro drug susceptibility assay are ...
    Evolution of Escherichia coli to Macrophage Cell Line
    Authors:  Migla Miskinyte and Isabel Gordo, date: 09/05/2014, view: 9094, Q&A: 0
    [Abstract] The genomes of species of Escherichia coli (E. coli) show an extraordinary amount of diversity, which include commensal strains and strains belonging to different pathovars. Many strains of E. coli, which can cause mild or severe pathologies in humans, have a commensal ancestor. Understanding the evolutionary changes ...
    Fitness Measurements of Evolved Esherichia coli
    Authors:  Migla Miskinyte and Isabel Gordo, date: 09/05/2014, view: 10332, Q&A: 0
    [Abstract] Bacteria can adapt very rapidly to novel selective pressures. In the transition from commensalism to pathogenicity bacteria have to face and adapt to the host immune system. Specifically, the antagonistic interaction imposed by one of the first line of defense of innate immunity cells, macrophages, on commensal bacteria, such as Escherichia coli ...
    In vitro DNA Polymerization Activity Assay Using Cell-free Extracts
    Authors:  Anurag K. Sinha and Malay K. Ray, date: 08/20/2014, view: 7413, Q&A: 0
    [Abstract] This protocol has been designed to measure the in-vitro DNA polymerization activity in crude cell extracts of the Antarctic bacterium Pseudomonas syrinagae Lz4W. This bacterium can grow at 4 °C with optimum growth rate at 22 °C. The slow growth rate of the bacterium observed at low temperature (4 °C) compared to higher ...
    Determination of Mutation Frequency During Viral DNA Replication
    [Abstract] This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. In the original Carrascosa et al. (1982), the protocol was carried out with two cloned ...

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