Microbiology

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    Protocols in Current Issue
    Quantification of HIV-2 DNA in Whole Blood
    Authors:  Zsófia Szojka, Sara Karlson, Marianne Jansson and Patrik Medstrand, date: 10/20/2019, view: 2350, Q&A: 0
    [Abstract] Time to AIDS infection is longer with HIV-2, compared to HIV-1, but without antiretroviral therapy both infections will cause AIDS-related mortality. In HIV-2 infection, monitoring of antiretroviral treatment (ART) efficacy is challenging since a large proportion of HIV-2-infected individuals displays low or undetectable plasma RNA levels. Hence, ...
    HBV Infection in Human Hepatocytes and Quantification of Encapsidated HBV DNA
    Authors:  Kai Li, Seiichi Sato and Akinori Takaoka, date: 01/20/2016, view: 8783, Q&A: 0
    [Abstract] Human hepatic cancer cell lines such as HepG2, Huh7, and HLE cannot get infected with Hepatitis B virus (HBV) due to lack of an HBV receptor(s). Transfection with HBV genome has so far been referred as a tool to mimic HBV infection. However, since sodium taurocholate cotransporting polypeptide (NTCP) was identified as a functional receptor for HBV ...
    Quantification of Total and 2-LTR (Long terminal repeat) HIV DNA, HIV RNA and Herpesvirus DNA in PBMCs
    Authors:  Marta Massanella, Sara Gianella, Steven M. Lada, Douglas D. Richman and Matthew C. Strain, date: 06/05/2015, view: 10330, Q&A: 0
    [Abstract] Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and understanding the association between asymptomatic replication of CMV (and other herpesvirus, including ...
    Quantification of HIV RNA and Human Herpesvirus DNA in Seminal Plasma
    Authors:  Milenka V. Vargas-Meneses, Marta Massanella, Caroline C. Ignacio and Sara Gianella, date: 05/05/2015, view: 7801, Q&A: 0
    [Abstract] Multiple viruses can co-infect the genital tract, modifying the immunologic and virologic milieu and possibly playing a role in viral transmission and pathogenesis. The aim of our studies has been to understand the complex relationships between HIV-1 RNA, and multiple human herpesviruses known to frequently replicate in the genital tract of ...
    Quantification of HIV-1 DNA
    Authors:  Ferdinand Roesch and Olivier Schwartz, date: 04/05/2013, view: 9980, Q&A: 0
    [Abstract] The reverse transcription (RT) reaction is a critical step in HIV-1 life cycle. It is very strongly regulated and the target of several restriction factors (TRIM5α, APOBECs, SAMHD1, etc.). The progress of reverse transcription can be followed by measuring viral DNA by quantitative PCR (qPCR). This method is sensitive enough to allow detection of ...



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