Cell Biology

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    Protocols in Current Issue
    Assessment of Human Dendritic Cell Antigen Uptake by Flow Cytometry
    Authors:  Ana Luque, Sonia Cárdenas-Brito, Rut Olivar and Josep M. Aran, date: 11/20/2013, view: 14848, Q&A: 0
    [Abstract] Antigen uptake by dendritic cells is the first key step towards induction of antigen-specific T-cell responses. This flow cytometry-based protocol describes the analysis of dendritic cell uptake of soluble antigens through two different mechanisms: non-specific macropinocytosis (using Lucifer Yelloy CH), and receptor-mediated endocytosis (using DQ ...
    Epidermal Growth Factor (EGF) Receptor Endocytosis Assay in A549 Cells
    Authors:  Sabrina Rizzolio and Luca Tamagnone, date: 08/05/2013, view: 16440, Q&A: 0
    [Abstract] The following endocytosis assay has been optimized to assess EGF-stimulated EGFR endocytosis; but could be modified to assess other ligand-stimulated endocytosis of plasma membrane receptors (for which fluorochrome-conjugated ligands are available to track their receptor internalization). In brief, cells are treated with fluorescent EGF at 4 °C to ...



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