Biochemistry

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    Protocols in Current Issue
    Electrophoresis Mobility Shift Assay
    Authors:  Masaru Nakata and Masaru Ohme-Takagi, date: 04/05/2014, view: 12574, Q&A: 0
    [Abstract] Protein (transcription factors and/or transcription cofactors)-binding to DNA is a critical event in regulation of transcription. Electrophoresis Mobility Shift Assay (EMSA), also known as gel shift assay, is a useful tool to detect protein- or protein complex-DNA/RNA interaction and to evaluate DNA binding specificity of transcription factors in ...
    EMSA Analysis of DNA Binding By Rgg Proteins
    Authors:  Breah LaSarre and Michael J. Federle, date: 08/05/2013, view: 10703, Q&A: 0
    [Abstract] In bacteria, interaction of various proteins with DNA is essential for the regulation of specific target gene expression. Electrophoretic mobility shift assay (EMSA) is an in vitro approach allowing for the visualization of these protein-DNA interactions. Rgg proteins comprise a family of transcriptional regulators widespread among the ...



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