Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
[Abstract] Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and ...
Determining Ribosome Translational Status by Ribo-ELISA
[Abstract] The Ribo-ELISA was originally developed to elucidate the basis for the ribopuromycylation method (RPM)-based detection of ribosome bound nascent chains. The Ribo-ELISA enables characterization of the translational status of ribosomes, and can be applied to the discovery of super-ribosomal complexes with novel ribosome associated macromolecules ...
Mitochondrial Biogenesis Assay after 5-day Treatment in PC-3 Cells
Authors: Yili Xu and
Joy Y. Feng,
date: 01/20/2015,
view: 7234,
Q&A: 0
[Abstract] Drug-induced mitochondrial injury can be caused by many different mechanisms including inhibition of mitochondrial DNA replication, transcription, translation, and altered protein function. Determination of the level of mitochondrial protein synthesis, or mitochondrial biogenesis, relative to the cellular protein synthesis, provides important ...
Competitive ELISA for Protein-lipopolysaccharide (LPS) Binding
[Abstract] Lipopolysaccharide is the major constituent of the outer membrane of gram-negative bacteria and, once released from the bacterial surface into the bloodstream, is a potent activator of the host immune system, which can lead to septic shock. LPS has a hydrophilic region consisting of a repeating oligosaccharide that is strain-specific (O-antigen) ...
In vitro Inflammasome Assay
Authors: Mario M. Zaiss and
Kendle M. Maslowski,
date: 06/05/2014,
view: 8759,
Q&A: 0
[Abstract] Innate immune cells sense pathogen and danger-associated molecular patterns (PAMPs and DAMPs) through a range of innate immune pattern recognition receptors (PRRs). One type of PRRs are the Nod-like receptors (NLRs), which form inflammasomes; a molecular platform required for the recruitment and activation of Caspase-1, which in turn cleaves and ...
ELISA on Virus-Infected Cells
Authors: Daniel L. Glauser and
Philip G. Stevenson,
date: 05/20/2014,
view: 6409,
Q&A: 0
[Abstract] The gammaherpesvirus murid herpesvirus 4 (MuHV-4) enters cells by endocytosis from the cell surface and fusion of the viral envelope with the membrane of late endosomes. The viral envelope glycoproteins undergo antigenic changes both upon virion endocytosis and upon fusion of the viral envelope with the endosomal membrane. These changes in virion ...
Chikungunya ELISA Protocol
[Abstract] This protocol will result in the accurate qualitative measurement of anti-Chikungunya virus antibody (Ab) from infected mouse tissue or serum. This assay was developed by Dr. Caitlin Briggs, Arbovax, Inc. Chikungunya is a BL3 agent and should be handled in a biosafety level 3 laboratory under BL3 conditions. This protocol was used in the ...