Cancer Biology

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    Protocols in Current Issue
    In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
    Authors:  Shin-Ae Kang, Sandra Bajana and Takemi Tanaka, date: 02/20/2016, view: 9627, Q&A: 1
    [Abstract] Hematogenous metastasis is a primary cause of mortality from metastatic cancer. The shear-resistant adhesion of circulating tumor cells to the vascular endothelial cell surface under blood flow is an essential step in cell extravasation and further tissue invasion. This is similar to a process exploited by leukocytes for adhesion to inflamed blood ...
    Hanging Drop Aggregation Assay of Breast Cancer Cells
    Author:  Yong Teng, date: 02/05/2015, view: 15016, Q&A: 0
    [Abstract] Hanging drop assay can be used to investigate cell-cell cohesion and cell-substratum adhesion through generation of 3D spheroids under physiological conditions. It also can be used to elucidate the role of cell-cell or cell-ECM interactions in specifying spatial relationships between two (or more) different cell populations. This simple method ...



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