Cancer Biology

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    Protocols in Current Issue
    Soft Agar Anchorage-independent Assay
    Authors:  Li-Ting Wang and Shih-Hsien Hsu, date: 10/20/2013, view: 12030, Q&A: 2
    [Abstract] Chronic inflammation drives initiation of hepatocellular carcinoma (HCC), but the underlying mechanisms linking inflammation and tumor formation remain obscure. In this study, soft agar anchorage-independent assay were used to determine tumor transform activity of hepatoma cells with ISX over expression or knockdown in vitro.
    Protocol for Apoptosis Assay by Flow Cytometry Using Annexin V Staining Method
    Authors:  Imayavaramban Lakshmanan and Surinder K Batra, date: 03/20/2013, view: 26489, Q&A: 1
    [Abstract] This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium iodide solution followed by flow cytometry analysis. It is based on the principle that normal cells are hydrophobic in nature as they express phosphatidyl serine in the inner ...
    Sphere Formation (Osteosphere/Sarcopshere) Assay
    Authors:  Upal Basu-Roy, Claudio Basilico and Alka Mansukhani, date: 12/20/2012, view: 18197, Q&A: 0
    [Abstract] Self-renewing cells from adult tissue (such as bone) that represent a progenitor population can be grown in suspension cultures in the presence of defined serum-free medium. Progenitor cells can be identified by this property of anchorage-independent growth in suspension cultures. These spherical clusters of progenitor bone cells growing under ...



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